Preparation of Fluorescent Tubulin Binders (pages 222–226)
Dr. Elena Riva, Dr. Martin Mattarella, Dr. Stella Borrelli, Dr. Michael S. Christodoulou, Dr. Daniele Cartelli, Dr. Marcus Main, Dr. Stephen Faulkner, Dr. Daniel Sykes, Prof. Dr. Graziella Cappelletti, Prof. Dr. John S. Snaith and Prof. Dr. Daniele Passarella
Article first published online: 14 JAN 2013 | DOI: 10.1002/cplu.201200260
A chip off the old block: Thiocolchicine, taxol and cephalomannine have been used as building blocks for the preparation of four different fluorescent compounds designed to image the tubulin/microtubule network in cells (see figure). Thiocolchicine gave the best results and the compound derived from conjugation with fluorescein minimally inhibits tubulin polymerization, is cell permeable and binds microtubules.