UNIT 16.16 Preparation of Cell Cultures and Vaccinia Virus Stocks

  1. Catherine A. Cotter,
  2. Patricia L. Earl,
  3. Linda S. Wyatt,
  4. Bernard Moss

Published Online: 5 JAN 2017

DOI: 10.1002/cpmb.33

Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

How to Cite

Cotter, C.A., Earl, P.L., Wyatt, L.S., and Moss, B. 2017. Preparation of cell cultures and vaccinia virus stocks. Curr. Protoc. Mol. Biol. 117:16.16.1-16.16.18. doi: 10.1002/cpmb.33

Author Information

  1. Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 5 JAN 2017


The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2017 by John Wiley & Sons, Inc.


  • cell culture;
  • vaccinia virus;
  • modified vaccinia virus Ankara