UNIT 16.16 Preparation of Cell Cultures and Vaccinia Virus Stocks
Published Online: 5 JAN 2017
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Molecular Biology
How to Cite
2017. Preparation of cell cultures and vaccinia virus stocks. Curr. Protoc. Mol. Biol. 117:16.16.1-16.16.18. doi: 10.1002/cpmb.33, , , and
- Published Online: 5 JAN 2017
The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2017 by John Wiley & Sons, Inc.
- cell culture;
- vaccinia virus;
- modified vaccinia virus Ankara