Unit

UNIT 3.30 Electroporation in the Rodent Embryonic Brain Using Whole Embryo Culture System

  1. Takako Kikkawa1,
  2. Masanori Takahashi2,3,
  3. Noriko Osumi1

Published Online: 3 JAN 2017

DOI: 10.1002/cpns.21

Current Protocols in Neuroscience

Current Protocols in Neuroscience

How to Cite

Kikkawa, T., Takahashi, M., and Osumi, N. 2017. Electroporation in the rodent embryonic brain using whole embryo culture system. Curr. Protoc. Neurosci. 78:3.30.1-3.30.16. doi: 10.1002/cpns.21

Author Information

  1. 1

    Department of Developmental Neuroscience, United Center for Advanced Research and Translational Medicine (ART), Tohoku University School of Medicine, Sendai, Miyagi, Japan

  2. 2

    Jichi Medical University Graduate School of Medicine, Shimotsuke, Tochigi, Japan

  3. 3

    Division of Biology, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan

Publication History

  1. Published Online: 3 JAN 2017

Abstract

This unit describes basic methods for mammalian whole embryo culture (WEC) using embryonic day 10.5 mouse embryos, including the preparation of high-quality immediately centrifuged (IC) rat serum that is commonly used for WEC and is essential for normal growth and development of cultured mouse and rat embryos in vitro. An alternative protocol for different stages of rodent embryos is also introduced. Since embryos for WEC are dissected out of the uterus and manipulated under the microscope, one can overcome many of the difficulties of gene delivery encountered using in utero electroporation. A description for a gene transfer method to label neural stem/progenitor cells of the cortical primordium in a highly region-specific manner is also included. © 2017 by John Wiley & Sons, Inc.

Keywords:

  • whole embryo culture;
  • electroporation;
  • brain development