Unit
UNIT 20.2 Measuring Protein Interactions by Optical Biosensors
Published Online: 3 APR 2017
DOI: 10.1002/cpps.31
Copyright © 2013 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Protein Science
Additional Information
How to Cite
, , & (2017). Measuring protein interactions by optical biosensors. Current Protocols in Protein Science, 88, 20.2.1–20.2.25. doi: 10.1002/cpps.31
Publication History
- Published Online: 3 APR 2017
Abstract
This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics of reversible protein interactions. Emphasis is placed on description of robust approaches that will provide reliable results with few assumptions. How to avoid the most commonly encountered problems and artifacts is also discussed. © 2017 by John Wiley & Sons, Inc.
Keywords:
- protein interactions;
- surface plasmon resonance;
- biolayer interferometry;
- optical biosensor
