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Exocellulase Activity of Cellobiohydrolase Immobilized on DNA-Wrapped Single-Walled Carbon Nanotubes

Authors

  • Jin Hee Kim,

    1. Department of Environmental Science and Technology, Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano 380-8553 (Japan)
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  • Prof. Masakazu Kataoka,

    1. Department of Environmental Science and Technology, Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano 380-8553 (Japan)
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  • Akio Kumagai,

    1. Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano 380-8553 (Japan)
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  • Hiroto Nishijima,

    1. Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano 380-8553 (Japan)
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  • Prof. Yoshihiko Amano,

    1. Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano 380-8553 (Japan)
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  • Prof. Yoong Ahm Kim,

    Corresponding author
    1. Department of Electrical and Electronic Engineering, Faculty of Engineering, Shinshu University, Wakasato, Nagano 380-8553 (Japan), Fax: (+81) 26-269-5208
    • Department of Electrical and Electronic Engineering, Faculty of Engineering, Shinshu University, Wakasato, Nagano 380-8553 (Japan), Fax: (+81) 26-269-5208
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  • Prof. Morinobu Endo

    1. Department of Electrical and Electronic Engineering, Faculty of Engineering, Shinshu University, Wakasato, Nagano 380-8553 (Japan), Fax: (+81) 26-269-5208
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Abstract

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Wrapped around the finger: A hybrid-type biocatalyst suitable for extreme conditions is engineered by immobilizing cellulase on DNA-wrapped single-walled carbon nanotubes (SWNTs). The exocellulases are attached to the bare surface of the nanotubes, which are helically wrapped with DNA. Such spatially hybridized cellulases on DNA-dispersed individual SWNTs are stable in alkaline conditions and at high temperatures and exhibit a high initial enzymatic activity.

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