These authors contributed equally to this work.
A Laminar-Flow Microfluidic Device for Quantitative Analysis of Microbial Electrochemical Activity
Article first published online: 5 JUN 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Special Issue: Microbial Fuel Cells
Volume 5, Issue 6, pages 1119–1123, June 2012
How to Cite
Li, Z., Venkataraman, A., Rosenbaum , M. A. and Angenent, L. T. (2012), A Laminar-Flow Microfluidic Device for Quantitative Analysis of Microbial Electrochemical Activity. ChemSusChem, 5: 1119–1123. doi: 10.1002/cssc.201100736
- Issue published online: 5 JUN 2012
- Article first published online: 5 JUN 2012
- Manuscript Received: 16 NOV 2011
- National Science Foundation. Grant Number: 0939882
- Chinese Scholarship Council
- National Science Foundation. Grant Number: ECS-0335765
- microbial electrochemical activity;
We report a laminar flow-based microfluidic bioelectrochemical system (BES) that was fabricated by using polydimethyl siloxane (PDMS) channels and gold electrodes. The microfluidic BES was operated as a potentiostatically controlled two-electrode system. A pure culture of Geobacter sulfurreducens strain PCA, which is a model electrode-respiring bacterium, was grown in the channel and respired with the electrode under strict anaerobic conditions. We took advantage of the short hydraulic retention time (≈2 min) and response times (<21 min) to rapidly test the effect of certain chemical stimuli, such as O2 and anthraquinone disulfide (AQDS), on electric current production by G. sulfurreducens. The results showed that: i) short-term (2 min) exposure to O2-saturated solution did not cause any irreversible toxicity to G. sulfurreducens, and ii) AQDS can be used as a redox mediator by G. sulfurreducens for shuttling electrons between the microbe and the electrode. We, therefore, demonstrate that the microfluidic BES is a promising research tool for gaining insight into microbial electrochemical activity. In our two-dimensional microfluidic-based research tool, a well-defined electrochemical environment can be maintained with the help of laminar flow without a membrane to separate two electrodes.