Biological Production of 2-Butanone in Escherichia coli

Authors

  • Hisanari Yoneda,

    1. Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616 (USA), Fax: (+1) 530-752-8995
    2. Central R&D laboratories, New Business Development, Asahi Kasei Corporation, 2-1 Samejima, Fuji, Shizuoka 416-8501 (Japan)
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  • Prof. Dean J. Tantillo,

    1. Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616 (USA), Fax: (+1) 530-752-8995
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  • Prof. Shota Atsumi

    Corresponding author
    1. Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616 (USA), Fax: (+1) 530-752-8995
    • Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, CA 95616 (USA), Fax: (+1) 530-752-8995

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Abstract

An Escherichia coli (E. coli) strain was engineered to synthesize 2-butanone from glucose by extending the 2,3-butanediol synthesis reaction sequence catalyzed by exogenous enzymes. To convert 2,3-butanediol to 2-butanone, B12-dependent glycerol dehydratase from Klebsiella pneumoniae was introduced into E. coli. It has been proposed that the enzyme has a weak activity toward 2,3-butanediol. The activity in E. coli is confirmed in this study. Furthermore, co-expressing coenzyme B12 reactivators increased the 2-butanone titer. This demonstration of 2-butanone production by extending the 2,3-butanediol biosynthetic pathway provides the possibility to produce this valuable chemical renewably.

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