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Fractionation of Hemp Hurds by Organosolv Pretreatment and its Effect on Production of Lignin and Sugars

Authors

  • Stefano Gandolfi,

    1. Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, Via Mario Bianco 9, 20131, Milano (Italy)
    2. The Protein Factory, Centro Interuniversitario di Biotecnologie Proteiche, Università degli Studi dell'Insubria, Politecnico di Milano, ICRM CNR, Milano (Italy)
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  • Dr. Gianluca Ottolina,

    1. Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, Via Mario Bianco 9, 20131, Milano (Italy)
    2. The Protein Factory, Centro Interuniversitario di Biotecnologie Proteiche, Università degli Studi dell'Insubria, Politecnico di Milano, ICRM CNR, Milano (Italy)
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  • Dr. Roberto Consonni,

    1. Istituto per lo Studio delle Macromolecole, Consiglio Nazionale delle Ricerche, Via Bassini 15, 20133, Milano (Italy)
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  • Dr. Sergio Riva,

    1. Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, Via Mario Bianco 9, 20131, Milano (Italy)
    2. The Protein Factory, Centro Interuniversitario di Biotecnologie Proteiche, Università degli Studi dell'Insubria, Politecnico di Milano, ICRM CNR, Milano (Italy)
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  • Dr. Ilabahen Patel

    Corresponding author
    1. Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, Via Mario Bianco 9, 20131, Milano (Italy)
    2. The Protein Factory, Centro Interuniversitario di Biotecnologie Proteiche, Università degli Studi dell'Insubria, Politecnico di Milano, ICRM CNR, Milano (Italy)
    • Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, Via Mario Bianco 9, 20131, Milano (Italy)===

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Abstract

Fractionation of hemp hurds into its three main components, cellulose, hemicellulose, and lignin, was carried out using organosolv pretreatment. The effect of processing parameters, such as temperature, catalyst concentration, reaction time, and methanol (MeOH) concentration, on the dissolution and recovery of hemicellulose and lignin was determined. More than 75 % of total hemicellulose and 75 % of total lignin was removed in a single step with low amounts of degradation products under the following conditions: 165 °C, 3 % H2SO4, 20 min reaction time, and 45 % MeOH. Enzymatic hydrolysis of the residual pretreated biomass yielded up to 60 % of cellulose-to-glucose conversion. The maximum recovery of the main components was obtained at a combined severity factor value of around one. Characterization of pretreated biomass and isolated lignin was carried out with FTIR and 2D 13C–1H correlation HSQC NMR spectroscopy, the latter technique providing detailed structural information about the obtained methanol organosolv lignin (MOSL). Results suggested that xylopyranoside is the major carbohydrate associated with hemp lignin. The chemical properties of MOSL samples in terms of their phenolic group content and antioxidant capacity were also investigated. The results showed that MOSL samples have a high phenolic group content and antioxidant capacity relative to Klason lignin.

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