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Long wavelength fluorophores and cell-by-cell correction for autofluorescence significantly improves the accuracy of flow cytometric energy transfer measurements on a dual-laser benchtop flow cytometer

  1. Zsolt Sebestyén1,
  2. Péter Nagy2,
  3. Gábor Horváth1,
  4. György Vámosi2,
  5. Reno Debets3,
  6. Jan W. Gratama3,
  7. Denis R. Alexander4,
  8. János Szöllősi1,*

Article first published online: 27 JUN 2002

DOI: 10.1002/cyto.10121

Issue

Cytometry

Cytometry

Volume 48, Issue 3, pages 124–135, 1 July 2002

Additional Information

How to Cite

Sebestyén, Z., Nagy, P., Horváth, G., Vámosi, G., Debets, R., Gratama, J. W., Alexander, D. R. and Szöllősi, J. (2002), Long wavelength fluorophores and cell-by-cell correction for autofluorescence significantly improves the accuracy of flow cytometric energy transfer measurements on a dual-laser benchtop flow cytometer. Cytometry, 48: 124–135. doi: 10.1002/cyto.10121

Author Information

  1. 1

    Department of Biophysics and Cell Biology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary

  2. 2

    Cell Biophysics Workgroup of the Hungarian Academy of Sciences, University of Debrecen, Debrecen, Hungary

  3. 3

    Department of Medical Oncology, Clinical and Tumor Immunology, University Hospital Rotterdam–Daniel den Hoed Cancer Center, Rotterdam, The Netherlands

  4. 4

    Laboratory of Lymphocyte Signalling and Development, Programme of Molecular Immunology, The Babraham Institute, Cambridge, United Kingdom

*Department of Biophysics and Cell Biology, Faculty of Medicine, Medical and Health Science Center, University of Debrecen, Nagyerdei kert 98, H-4012 Debrecen, Hungary

Publication History

  1. Issue published online: 27 JUN 2002
  2. Article first published online: 27 JUN 2002
  3. Manuscript Accepted: 1 MAY 2002
  4. Manuscript Revised: 15 APR 2002
  5. Manuscript Received: 1 FEB 2002

Funded by

  • European Commission. Grant Number: FP 5 QLRT-1999-3126
  • Hungarian–British Joint Academic and Research Programme. Grant Number: 069
  • Hungarian Academy of Sciences. Grant Number: OTKA 303999
  • Hungarian Ministry of Health. Grant Numbers: ETT 07-461, ETT 117/2001
  • Hungarian Ministry of Education. Grant Number: FKFP 0607

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