Original Articles
You have full text access to this OnlineOpen article
Enumeration of CD34+ cells in cord blood: A variation on a single-platform flow cytometric method based on the ISHAGE gating strategy
Article first published online: 14 AUG 2001
DOI: 10.1002/cyto.1136
Copyright © 2001 Wiley-Liss, Inc.
Additional Information
How to Cite
Brocklebank, A. M. and Sparrow, R. L. (2001), Enumeration of CD34+ cells in cord blood: A variation on a single-platform flow cytometric method based on the ISHAGE gating strategy. Cytometry, 46: 254–261. doi: 10.1002/cyto.1136
Publication History
- Issue published online: 26 DEC 2002
- Article first published online: 14 AUG 2001
- Manuscript Accepted: 8 MAY 2001
- Manuscript Received: 27 MAR 2001
LITERATURE CITED
- 1, . Placental and/or umbilical cord blood: an alternative source of hematopoietic stem cells for transplantation. Blood 1997; 90: 4665–4678.
- 2, , , , , , , , , , , . Outcomes among 562 recipients of placental-blood transplants from unrelated donors. N Engl J Med 1998; 339: 1565–1577.
- 3. Current status of umbilical cord blood hematopoietic stem cell transplantation. Exp Hematol 2000; 28: 1197–1205.
- 4, , , , . Sensitive detection and enumeration of CD34+ cells in peripheral and cord blood by flow cytometry. Exp Hematol 1994; 22: 1003–1010.
- 5, , , , , , , , , , , , , , , . Flow cytometric enumeration of CD34+ hematopietic stem and progenitor cells. Cytometry 1998; 34: 128–142.
- 6, , , , , , , , , , , , , , . Cytofluorometric methods for assessing absolute numbers of cell subsets in blood. Cytometry 2000; 42: 327–346.
- 7, . Stem cell enumeration by flow cytometry: current concepts and recent developments in CD34+ cell enumeration. Cytotherapy 2000; 2: 395–402.
- 8
- 9, , , , , . Minimization of CD34+ cell enumeration variability using the ProCOUNT standardized methodology. J Hematother 1998; 7: 499–504.
- 10, , , , , , , , . Enumeration of CD34-positive stem cells: evaluation and comparison of three methods. J Hematother 1997; 6: 213–226.
- 11, , , , , , . Semi-automated flow cytometric analysis of CD34-expressing hematopoietic cells in peripheral blood progenitor cell apheresis products. Transfusion 1999; 39: 1220–1226.
- 12, , , . Single- versus dual-platform assays for human CD34+ cell enumeration. Cytometry 1999; 38: 274–279.
- 13, , , , , , , , . Measurement of absolute concentration and viability of CD34+ cells in cord blood and cord blood products using fluorescent beads and cyanine nucleic acid dyes. Cytometry 1998; 34: 121–127.
- 14, , . Flow cytometry of CD34+ cells in whole blood. Nature Med 2000; 6: 833–836.
- 15, , , , , . Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. Cytometry 1998; 34: 61–70.
- 16, , , , , , . Reduction of intra- and interlaboratory variation in CD34+ stem cell enumeration using a stable test material, standard protocols and targeted training. Br J Haematol 2000; 108: 784–792.
- 17, . Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986; 1: 307–310.
- 18
- 19, , . Comparative analysis of whole blood lysis methods for flow cytometry. Cytometry 1997; 30: 124–133.
- 20, , , , , . The influence of different erythrocyte lysing procedures on flow cytometric determination of CD34+ cells in umbilical cord blood transplants. Transfusion Med 1998; 8: 111–118.
- 21, , , . Selective susceptibility of CD34-expressing cells to acquire flow cytometric features of apoptosis/necrosis on exposure to an ammonium chloride-based red blood cell lysing reagent. J Hematother 1998; 7: 315–318.
- 22, , , , , , , , , , , . Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry 1998; 34: 264–271.
- 23, , , . Effect of fixatives on CD34+ cell enumeration. J Hematother Stem Cell Res 1999; 8: 327–329.
- 24, , , . Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erythrocyte lysing reagents. J Immunol Methods 2000; 239: 13–23.
- 25, , . An automated cell counter compared with flow cytometry for obtaining accurate leukocyte and lymphocyte subset estimations. Transfusion Med 1998; 8: 157.
- 26, , , . The “vanishing counting bead” phenomenon: effect on absolute CD34+ cell counting in phosphate-buffered saline-diluted leukapheresis samples. Cytometry 2001; 43: 154–160.

1552-4930/asset/olbannerleft.gif?v=1&s=cf6fcbfdc8ffa722551b71f8be0977b5b04db6ee)
1552-4930/asset/olbannerright.gif?v=1&s=6d0ba4e64946e5d9d4ebba4b8395a68bf324cd0b)
1552-4930/asset/cover.gif?v=1&s=3df33f34517aaa739d7876df137e2ab92c5f82e7)