Differential of light-scattering detection in an arc-lamp-based epi-illumination flow cytometer
Article first published online: 30 MAR 2005
Copyright © 1985 Wiley-Liss, Inc.
Volume 6, Issue 4, pages 281–285, July 1985
How to Cite
Steen, H. B. and Lindmo, T. (1985), Differential of light-scattering detection in an arc-lamp-based epi-illumination flow cytometer. Cytometry, 6: 281–285. doi: 10.1002/cyto.990060402
- Issue published online: 16 JUN 2005
- Article first published online: 30 MAR 2005
- Manuscript Accepted: 5 MAR 1985
- Manuscript Received: 5 DEC 1984
- Flow cytometry;
- light scattering;
- blood cells
Light-scattering histograms of blood cell suspensions were recorded for various ranges of scattering angles by means of an arc-lamp-based flow cytometer (AL-FCM). The results were compared with those obtained with a conventional, laser-based flow cytometer (L-FCM) with forward scattering (2–20°) and scattering at right angles. Measuring with the AL-FCM in the angle range upward from 13°, the relative light-scattering intensities of lymphocytes, monocytes, and granulocytes were essentially independent of scattering angle and closely similar to the values measured as right-angle scattering in the L-FCM. With a range of scattering angles upward from 2° the AL-FCM yielded histograms similar although not identical to that of the forward-scattering detector in the L-FCM. Differentiation between live and dead cells in this mode of operation was similar in the two instruments.