The LLNL high-speed sorter: Design features, operational characteristics, and biological utility

Authors


  • Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48 with support from HHS grants CA 30613 (E.B.) and CA 31714 (E.B.) and the National Laboratory Gene Library Project. A preliminary report on portions of this instrument has been given in the Department of Energy publication: Energy and Technology Review, UCRL-52000-84-3, 1984, pp 13–21.

  • Presented at the Conference on Analytical Cytology X, Asilomar Conference Grounds, Pacific Grove, California, June 3–8, 1984.

Abstract

This report describes a dual-beam, high-speed sorter (HiSS) with a droplet production rate of 220,000 s−1 now in routine use at the Lawrence Livermore National Laboratory. The system can process and sort objects at rates in excess of 20,000 s−1. We report here on the development of HiSS, describe its operational characteristics, and evaluate its utility for analysis and purification of human chromosomes, human erythrocytes, and living Chinese hamster ovary cells.

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