Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W-7405-ENG-48 with support from HHS grants CA 30613 (E.B.) and CA 31714 (E.B.) and the National Laboratory Gene Library Project. A preliminary report on portions of this instrument has been given in the Department of Energy publication: Energy and Technology Review, UCRL-52000-84-3, 1984, pp 13–21.
Article first published online: 30 MAR 2005
Copyright © 1985 Wiley-Liss, Inc.
Volume 6, Issue 4, pages 290–301, July 1985
How to Cite
Peters, D., Branscomb, E., Dean, P., Merrill, T., Pinkel, D., Van Dilla, M. and Gray, J. W. (1985), The LLNL high-speed sorter: Design features, operational characteristics, and biological utility. Cytometry, 6: 290–301. doi: 10.1002/cyto.990060404
Presented at the Conference on Analytical Cytology X, Asilomar Conference Grounds, Pacific Grove, California, June 3–8, 1984.
- Issue published online: 16 JUN 2005
- Article first published online: 30 MAR 2005
- Manuscript Accepted: 21 FEB 1985
- Manuscript Received: 17 JAN 1985
- High-speed sorting;
- dual-beam flow cytometry;
- chromosome sorting;
- cell sorting
This report describes a dual-beam, high-speed sorter (HiSS) with a droplet production rate of 220,000 s−1 now in routine use at the Lawrence Livermore National Laboratory. The system can process and sort objects at rates in excess of 20,000 s−1. We report here on the development of HiSS, describe its operational characteristics, and evaluate its utility for analysis and purification of human chromosomes, human erythrocytes, and living Chinese hamster ovary cells.