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Keywords:

  • High-speed sorting;
  • dual-beam flow cytometry;
  • chromosome sorting;
  • cell sorting

Abstract

This report describes a dual-beam, high-speed sorter (HiSS) with a droplet production rate of 220,000 s−1 now in routine use at the Lawrence Livermore National Laboratory. The system can process and sort objects at rates in excess of 20,000 s−1. We report here on the development of HiSS, describe its operational characteristics, and evaluate its utility for analysis and purification of human chromosomes, human erythrocytes, and living Chinese hamster ovary cells.