A method to measure the duration of DNA syntheses and the potential doubling time from a single sample
Version of Record online: 8 MAR 2005
Copyright © 1985 Wiley-Liss, Inc.
Special Issue: Monoclonal Antibodies Against Bromodeoxyuridine
Volume 6, Issue 6, pages 620–626, November 1985
How to Cite
Begg, A. C., McNally, N. J., Shrieve, D. C. and Kärche, H. (1985), A method to measure the duration of DNA syntheses and the potential doubling time from a single sample. Cytometry, 6: 620–626. doi: 10.1002/cyto.990060618
- Issue online: 16 JUN 2005
- Version of Record online: 8 MAR 2005
- Manuscript Accepted: 19 JUL 1985
- Manuscript Received: 28 FEB 1985
- monoclonal antibody;
- flow cytometry;
- cell kinetics
A method is described whereby the DNA synthesis time, Ts, call be calculated using data of a single sample of cells taken several hours after labelling with bromodeoxyuridine (BrdUrd). The method involves a simple calculation using flow cytometry data of BrdUrd incorporation (green fluorescence, FITC-labelled anti-BrdUrd-DNA antibody) and total DNA content (red fluorescence, propidium iodide). The movement of BrdUrd-labelled cells through the S phase can be Ouantified by measuring their mean red fluorescence relative to that of G1 and G2 cells. Assuming the movement of the labelled cells toward G2 is linear with time, T., can be calculated by measuring their relative movement at any one time. The method was tested on cells in vitro and on bone marrow and tumor cells in vivo. Reasonable agreement was seen with published estimates of TS for these tissues.