Simultaneous separate detection of low angle and large angle light scattering in an arc lamp-based flow cytometer
Article first published online: 8 MAR 2005
Copyright © 1986 Wiley-Liss, Inc.
Volume 7, Issue 5, pages 445–449, September 1986
How to Cite
Steen, H. B. (1986), Simultaneous separate detection of low angle and large angle light scattering in an arc lamp-based flow cytometer. Cytometry, 7: 445–449. doi: 10.1002/cyto.990070509
- Issue published online: 16 JUN 2005
- Article first published online: 8 MAR 2005
- Manuscript Accepted: 16 APR 1986
- Manuscript Received: 25 JUL 1985
- light scattering;
- arc lamp flow cytometer
A device is described for simultaneous separate detection of the light scattering of cells at low and large scattering angles in an arc lamp-based flow cytometer with epi-illumination through an oil immersion microscope objective. Light scattering was measured in a dark field configuration that allows separate detection of light scattering greater than 2° and 15°, respectively. Dual parameter light scattering histograms of a blood cell suspension containing various types of leukocytes were closely similar to that obtained with a commercial laser-based instrument with light scattering detection at forward and right angles. The sensitivity of the device was sufficient to measure polystyrene particles with 0.25-μm diameter. A potential application may be differentiation of bacteria.