A method to average immunofluorescent histograms

Authors

  • Dr. G. E. Marti,

    Corresponding author
    1. Hematology Service, Biomedical Engineering and Instrumentation Branch, Division of Research Services, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892
    • NIH, Bldg. 10, Rm. 2C390, Bethesda, MD 20892
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  • W. Schuette,

    1. Clinical Pathology Department, Clinical Center and Applied Clinical Engineering Section, Biomedical Engineering and Instrumentation Branch, Division of Research Services, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892
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  • L. Magruder,

    1. Hematology Service, Biomedical Engineering and Instrumentation Branch, Division of Research Services, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892
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  • H. R. Gralnick

    1. Hematology Service, Biomedical Engineering and Instrumentation Branch, Division of Research Services, Clinical Center, National Institutes of Health, Bethesda, Maryland 20892
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Abstract

Single parameter gated flow cytometric fluorescent histograms were obtained on normal donor blood mononuclear cells using several commonly available lymphocyte surface markers. A computer method was developed to average single parameter flow cytometric immunofluorescent histograms. The averaged histograms provide a means of pattern recognition for normal lymphocytes and will aid in the clinical evaluation of lymphocytosis. Averaged histograms may also serve as standards for more advanced analysis.

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