Specific nuclear elimination in polyploid plasmodia of the slime mold physarum polycephalum
Version of Record online: 8 MAR 2005
Copyright © 1988 Wiley-Liss, Inc.
Volume 9, Issue 3, pages 261–265, May 1988
How to Cite
Werenskiold, A. K., Schreckenbach, Th. and Valet, G. (1988), Specific nuclear elimination in polyploid plasmodia of the slime mold physarum polycephalum. Cytometry, 9: 261–265. doi: 10.1002/cyto.990090312
- Issue online: 16 JUN 2005
- Version of Record online: 8 MAR 2005
- Manuscript Accepted: 11 NOV 1987
- Manuscript Received: 27 JUL 1987
- heploid stain;
- cell growth
In growing plasmodia of the myxomycete Physarum polycephalum (G2-phase), three distinct classes of nuclei with a relative DNA content of 1x, 2x, and 4x are observed in the presumed haploid strain CL. The 2x and 4x species comprise up to 35% and 5% of the nuclei. Quantitative cytofluorometric studies of nuclei isolated in either G2- or S-phase or after FUDR treatment (G1 arrest) show that the three nuclear populations undergo a synchronous mitotic cycle and that the relative DNA content of the nuclear fractions in G-2 phase reflects the 2c, 4c, and 8c state.
The heterogeneity of the nuclear population does, however, seem to be restricted to the growth phase. During a starvation period of 4 days that always preceeds sporulation (and also meiosis), the 4c nuclear population is reduced to 7%, 8c nuclei are no longer detected. These results suggest that a mechanism exists in Physarum for the selective detection and elimination of polyploid nuclei.