Work performed under the auspices of the U.S. Department of Energy by the Lawrence Livermore National Laboratory under contract number W–7405–ENG–48, with support from NIH Grant HD 17665.
Improved resolution of flow cytometric measurements of hoechst- and chromomycin-A3-stained human chromosomes after addition of citrate and sulfite†
Article first published online: 8 MAR 2005
Copyright © 1988 Wiley-Liss, Inc.
Volume 9, Issue 3, pages 266–270, May 1988
How to Cite
Engh, G. V. D., Trask, B., Gray, J. and Lansdorp, P. (1988), Improved resolution of flow cytometric measurements of hoechst- and chromomycin-A3-stained human chromosomes after addition of citrate and sulfite. Cytometry, 9: 266–270. doi: 10.1002/cyto.990090313
- Issue published online: 16 JUN 2005
- Article first published online: 8 MAR 2005
- Manuscript Accepted: 16 DEC 1987
- Manuscript Received: 30 OCT 1987
- Flow karyotype;
- chronic myeloid leukemia
The resolution of bivariate flow karyotypes of human chromosomes stained with Hoechst 33258 and chromomycin A3 can be increased by adding sodium citrate and sodium sulfite to the chromosomes shortly before measurement. A flow karyotype of a patient with chronic myelocytic leukemia is shown to illustrate that the addition of these compounds allows high-resolution measurements to be made and evaluated reliably from clinical samples.