Multiple fluorescence in situ hybridization

Authors

  • Dr. P. M. Nederlof,

    Corresponding author
    1. Sylvius Laboratories, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
    • Sylvius Laboratories, Department of Cytochemistry, University of Leiden 2333 AL Leiden, The Netherlands
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  • S. van der Flier,

    1. Sylvius Laboratories, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
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  • J. Wiegant,

    1. Sylvius Laboratories, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
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  • A. K. Raap,

    1. Sylvius Laboratories, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
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  • H. J. Tanke,

    1. Sylvius Laboratories, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
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  • J. S. Ploem,

    1. Sylvius Laboratories, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
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  • M. van der Ploeg

    1. Sylvius Laboratories, Department of Cytochemistry and Cytometry, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands
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Abstract

A method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue.

This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation in multiple colors. The possibility to detect multiple targets simultaneously is important for prenatal diagnosis and the detection of numerical and/or structural chromosome aberrations in tumor diagnosis. It may form the basis for an in situ hybridization based chromosome banding technique.

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