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Proposed standard for image cytometry data files†

  1. Phillip Dean1,*,
  2. Laura Mascio1,
  3. David Ow1,
  4. Damir Sudar2,
  5. James Mullikin3

Article first published online: 8 MAR 2005

DOI: 10.1002/cyto.990110502

Issue

Cytometry

Cytometry

Volume 11, Issue 5, pages 561–569, 1990

Additional Information

How to Cite

Dean, P., Mascio, L., Ow, D., Sudar, D. and Mullikin, J. (1990), Proposed standard for image cytometry data files. Cytometry, 11: 561–569. doi: 10.1002/cyto.990110502

Author Information

  1. 1

    Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, California 94551

  2. 2

    Department of Laboratory Medicine, University of California, San Francisco, California 94143

  3. 3

    Pattern Recognition Group, Delft University of Technology, Delft, The Netherlands

*Biomedical Sciences Division, Lawrence Livermore National Laboratory, P.O. Box 5507 L-452, Livermore, CA 94551

  1. †

    This work was supported by the U.S. Department of Energy at the Lawrence Livermore National Laboratory under contract W-7405-ENG-48 (P.D., L.M., and D.O.), by the Program for Analytical Cytology (University of California-LLNL) (D.S.), and by the Netherlands Project Team for Computer Science Research, Spin (Project Three)-Dimensional Image Analysis (J.M.).

Publication History

  1. Issue published online: 21 JUN 2005
  2. Article first published online: 8 MAR 2005
  3. Manuscript Accepted: 13 MAR 1990
  4. Manuscript Received: 31 AUG 1989

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Keywords:

  • Analysis;
  • processing;
  • storage;
  • database

Abstract

A number of different types of computers running a variety of operating systems are presently used for the collection and analysis of image cytometry data. In order to facilitate the development of sharable data analysis programs, to allow for the transport of image cytometry data from one installation to another, and to provide a uniform and controlled means for including textual information in data files, this document describes a data storage format that is proposed as a standard for use in image cytometry. In this standard, data from an image measurement are stored in a minimum of two files. One file is written in ASCII to include information about the way the image data are written and optionally, information about the sample, experiment, equipment, etc. The image data are written separately into a binary file. This standard is proposed with the intention that it will be used internationally for the storage and handling of biomedical image cytometry data. The method of data storage described in this paper is similar to those methods published in American Association of Physicists in Medicine (AAPM) Report Number 10 and in ACR-NEMA Standards Publication Number 300-1985.

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