This work was supported by grants from the Austrian “Fonds zur Foerderung der wissenschaftlichen Forschung” (P6066F) and “Oesterreichische Krebsgesellschaft, Sektion Tirol.”
Flow cytometric determination of atypical antigen expression in acute leukemia for the study of minimal residual disease†
Version of Record online: 8 MAR 2005
Copyright © 1992 Wiley-Liss, Inc.
Volume 13, Issue 8, pages 893–901, 1992
How to Cite
Drach, J., Drach, D., Glassl, H., Gattringer, C. and Huber, H. (1992), Flow cytometric determination of atypical antigen expression in acute leukemia for the study of minimal residual disease. Cytometry, 13: 893–901. doi: 10.1002/cyto.990130813
- Issue online: 21 JUN 2005
- Version of Record online: 8 MAR 2005
- Manuscript Accepted: 8 MAY 1992
- Manuscript Received: 20 JAN 1992
- Double color flow cytometry;
- rare events
The aim of this study was to investigate to which extent acute leukemias could be monitored for residual disease by using atypical antigen combinations as leukemia-related markers. Atypical antigenic features were determined by double color flow cytometry and included coexpression of lymphoid and myeloid related antigens, unnphysiological coexpression of immature and mature antigens, and lack of an antigen that is normally expressed during maturation. Atypical immunophenotypes were detected in 35 of 68 patients with AML (51.5%) and 15 of 24 patients with ALL (62.5%). When 12 patients with leukemia-associated markers were again analyzed at relapse, the relevant antigen combinations were retained in 11 of them. The sensitivity of this two color flow cytometric assay as determined in dilution experiments was 1 in 103 to 104 cells
Follow-up studies of bone marrow samples revealed that, after induction chemotherapy cells with leukemia-associated markers were detectable in several patients at a frequency of 0.5 to 4%, but only patients in whom the cells with atypical antigens never disappeared suffered from relapse. In contrast, patients who became negative for the atypical cells remained in complete remission (median remission duration after the first negative bone marrow assessment by flow cytometry 52 weeks, range 20–102)
We conclude that atypical antigen combinations, which are present in a meaningful number of acute leukemias, are a valuable means of monitoring acute leukemia patients during follow-up. This flow cytometric approach can complement other strategies to get a more accurate definition of remission in acute leukemia. © 1992 Wiley-Liss, Inc.