Rhodamine 123 fluorescence of immortal hybridoma cell lines as a function of glucose concentration
Version of Record online: 8 MAR 2005
Copyright © 1993 Wiley-Liss, Inc.
Volume 14, Issue 1, pages 70–73, 1993
How to Cite
Borth, N., Kral, G. and Katinger, H. (1993), Rhodamine 123 fluorescence of immortal hybridoma cell lines as a function of glucose concentration. Cytometry, 14: 70–73. doi: 10.1002/cyto.990140112
- Issue online: 21 JUN 2005
- Version of Record online: 8 MAR 2005
- Manuscript Accepted: 6 JUL 1992
- Manuscript Received: 6 FEB 1992
- Flow cytometry;
- glucose consumption rates;
- mitochondrial activity;
- rhodamine 123
The fluorescence of rhodamine 123 stained cells has been described to specifically reflect the activity of mitochondria. Changes in the intensity of fluorescence observed in stimulated lymphocytes were attributed to an increased glycolytic activity of cells due to increased growth rates. Previously reported changes in mitochondrial activity observed in batch cultures were likewise attributed to changed growth rates. In this study we report that the Rh123 fluorescence of hybridoma cell lines in batch culture more closely correlates to the glucose concentration in the culture supernatant than to growth rates. When cells are transferred into glutamine free medium with defined glucose concentrations ranging from 0 to 3,000 mg/L the mean Rh123 fluorescence adapts to the respective glucose concentration within 6 hours and gives a linear correlation. This can be explained by the previously described dependence of specific glucose consumption rates on glucose availability in the medium. The importance of controlling glucose availability, especially in large scale fermentations, is discussed. © 1993 Wiley-Liss, Inc.