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Keywords:

  • Flow cytometry;
  • lymphocyte gating;
  • CD4+ T-cells;
  • multiparameter flow cytometry

Abstract

We evaluated a flow cytometrïc method for determining the proportion of CD4-positive T lymphocytes in whole blood using a single three-color tube containing fluorochrome-labeled CD45, CD3, and CD4. Various ways of gating this sample were evaluated and results were compared with data obtained in our standard six-tube, two-color assay gated on light scatter parameters. Excellent correlation was found between the three-color analysis using a CD45/side scatter gate and the standard two-color analysis with a light scatter gate. This single-tube three-color test is a promising assay for monitoring CD4+ T-lymphocytes. © 1993 Wiley-Liss, Inc.1