Evaluation of the number of positive cells from flow cytometric immunoassays by mathematical modeling of cellular autofluorescence
Article first published online: 8 MAR 2005
Copyright © 1994 Wiley-Liss, Inc.
Volume 15, Issue 4, pages 294–301, 1 April 1994
How to Cite
Lampariello, F. (1994), Evaluation of the number of positive cells from flow cytometric immunoassays by mathematical modeling of cellular autofluorescence. Cytometry, 15: 294–301. doi: 10.1002/cyto.990150404
- Issue published online: 23 JUN 2005
- Article first published online: 8 MAR 2005
- Manuscript Accepted: 8 OCT 1993
- Manuscript Received: 12 OCT 1992
- Flow cytometry;
- immunofluorescence histogram analysis;
- percentage of positive cells
A method, for automated evaluation of the percentage of positive cells from flow cytometric, immunofluorescence histograms is presented. The method is based on a suitable mathematical representation of cellular autofluorescence distribution which is used to identify the negative cell distribution in the test histogram. An algorithm is developed and implemented in a computer program that quantifies the positive cells from the routinely available control and test histograms. Its accuracy is compared to that of the other currently used automated methods of analysis on the basis of a set of histograms with overlapping distributions and with known percentages of positive cells, generated by artificial mixing of real data. © 1994 Wiley-Liss, Inc.