Abbreviations:LDH, lactic dehydrogenase; MTT, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide); PBS, phosphate-buffered saline.
Development of a method for measuring cell number: Application to CNS primary neuronal cultures†
Article first published online: 8 MAR 2005
Copyright © 1994 Wiley-Liss, Inc.
Volume 17, Issue 3, pages 274–276, 1 November 1994
How to Cite
Volonté, C., Ciotti, M. T. and Battistini, L. (1994), Development of a method for measuring cell number: Application to CNS primary neuronal cultures. Cytometry, 17: 274–276. doi: 10.1002/cyto.990170311
- Issue published online: 23 JUN 2005
- Article first published online: 8 MAR 2005
- Manuscript Accepted: 11 MAY 1994
- Manuscript Received: 19 JAN 1994
- CNS primary neurons;
- rat cerebellar granules;
- cellular quantification;
In the present work we further develop a method for counting cell number that is totally independent from the permeability or uptake conditions of the cells, from the state of activation of intracellular enzymes, and from cellular metabolism. We provide a visual characterization of the method and show that it is highly suitable for cells not growing as monolayers as well as for cultures containing numberous aggregates. We also extend the applicability of this method to CNS primary neuronal cultures and show its direct comparison with alternative means for cellular quantification. The technique is fast, does not require tedious procedures or long washes, and offers advantages such as a high sensitivity and no background. © 1994 Wiley-Liss, Inc.