Quality control in flow cytometry for diagnostic pathology: II. A conspectus of reference ranges for lymphocyte immunophenotyping

Authors

  • J. Philip McCoy Jr.,

    Corresponding author
    1. Flow Cytometry Laboratory, Division of Pediatric Hematology/Oncology, Cooper Hospital/UMC and the UMDNJ-Robert Wood Johnson Medical School, Camden, New Jersey
    • Flow Cytometry Laboratory, Three Cooper Plaza, Suite 410, Division of Pediatric Hem/Onc, Camden, NJ 08103
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  • W. Roy Overton

    1. Flow Cytometry Laboratory, Division of Pediatric Hematology/Oncology, Cooper Hospital/UMC and the UMDNJ-Robert Wood Johnson Medical School, Camden, New Jersey
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  • This work was funded by the house of kids, inc.

  • Second in a series. Part 1 can be found: McCoy Jp Jr, Carey jL, Krause JR: Quality Control in Flow Cytometry for Diagnostic Pathology: I. Phenotyping and General Laboratory Procedures. Am J Clin Pathol 93: [supp 1]S27–S37, 1990.

Abstract

Immunophenotyping, as many other clinical assays, is interpreted only in the context of reference values obtained from healthy control individuals. While the use of these reference values, or ranges, has been commonplace in the clinical flow cytometry laboratory for well over a decade, there has been little consensus in standardizing how these values should be obtained, analyzed, or expressed. This report reviews the variables to be considered in establishing reference ranges and statistical methods which can be used. Additionally, examples are given of previously published reference ranges for a variety of specimens often submitted for immunophenotyping. © 1994 Wiley-Liss, Inc.

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