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Spatial dependence of the optical collection efficiency in flow cytometry

  1. Peter M. Goodwin1,*,
  2. W. Patrick Ambrose1,
  3. John C. Martin2,
  4. Richard A. Keller1

Article first published online: 8 MAR 2005

DOI: 10.1002/cyto.990210205

Issue

Cytometry

Cytometry

Volume 21, Issue 2, pages 133–144, October 1995

Additional Information

How to Cite

Goodwin, P. M., Ambrose, W. P., Martin, J. C. and Keller, R. A. (1995), Spatial dependence of the optical collection efficiency in flow cytometry. Cytometry, 21: 133–144. doi: 10.1002/cyto.990210205

Author Information

  1. 1

    Chemical Science and Technology Division, Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, New Mexico

  2. 2

    Life Sciences Division, Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, New Mexico

*Los Alamos National Laboratory, P.O. Box 1663, Mail Stop M888, Los Alamos, NM 87545

Publication History

  1. Issue published online: 23 JUN 2005
  2. Article first published online: 8 MAR 2005
  3. Manuscript Accepted: 6 APR 1995
  4. Manuscript Received: 27 APR 1994

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Literature Cited

  • 1
    Ambrose WP, Goodwin PM, Jett JH, Johnson ME, Martin JC, Marrone BL, Schecker JA, Wilkerson CW, Keller RA: Application of single molecule detection to DNA sequencing and sizing. Ber Bunsenges Phys Chem 97(12): 1535–1542, 1993.
  • 2
    Ambrose WP, Goodwin PM, Martin JC, Keller RA: Single molecule detection and photochemistry on a surface using near-field optical excitation. Phys Rev Lett 72(1): 160–163, 1994.
  • 3
    Barnes MD, Ng KC, Whitten WB, Ramsey JM: Detection of single rhodamine 6G molecules in levitated microdroplets. Anal Chem 65(17): 2360–2365, 1993.
  • 4
    Benford JR: Microscope objectives. In: Applied Optics and Optical Engineering, Vol 3, KingslakeR (ed.) Academic Press, New York, 1965, pp 145–182.
  • 5
    Betzig E, Trautman JK, Harris TD, Weiner JS, Kostelak RL: Breaking the diffraction barrier: Optical microscopy on a nanometric scale. Science 251: 1468–1470, 1991.
  • 6
    Cagnet M, Françon M, Thrierr JC: Atlas of Optical Phenomena. Springer-Verlag. Berlin, 1962.
  • 7
    Clark SM, Mathies RA: High-speed parallel separation of DNA restriction fragments using capillary array electrophoresis. Anal Biochem 215: 163–170, 1993.
  • 8
    Dovichi NJ, Martin JC, Jett JH, Keller RA: Attogram detection limit for aqueous dye samples by laser induced fluorescence. Science 219: 845–847, 1983.
  • 9
    Goodwin PM, Johnson ME, Martin JC, Ambrose WP, Marrone BL, Jett JH, Keller RA: Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry. Nucleic Acids Res 21(4): 803–806, 1993.
  • 10
    Goodwin PM, Wilkerson CW, Ambrose WP, Keller RA: Ultrasensitive detection of single molecules in flowing sample streams by laserinduced fluorescence. In: Ultrasensitive Laboratory Diagnostics, Proc. SPIE, Vol 1895, CohnGE (ed.) International Society for Optical Engineering, Bellingham, 1993, pp 79–89.
  • 11
    Hahn JH, Soper SA, Nutter HL, Martin JC, Jett JH, Keller RA: Laserinduced fluorescence detection of rhodamine-6G at 6 × 10−15 M. Appl Spectrosc 45(5): 743–746, 1991.
  • 12
    Horowitz P, Hill W: The Art of Electronics, Ed. 2. Cambridge University Press, Cambridge, 1989, p 184.
  • 13
    Huang XC, Quesada MA, Mathies RA: Capillary array electrophoresis using laser-excited confocal fluorescence detection. Anal Chem 64: 967–972, 1992.
  • 14
    Kachel V, Fellner-Feldegg H, Menke E: Hydrodynamic properties of flow cytometry instruments. In: Flow Cytometry and Sorting. Ed. 2, MelamedMR, LindmoT, MendelsohnML (eds.) John Wiley & Sons, New York, 1990, pp 27–44.
  • 15
    Kubin RF, Fletcher AN: Fluorescence quantum yields of some rhodamine dyes. J Lumin 27: 455–462, 1982.
  • 16
    Lee YH, Maus RG, Smith BW, Winefordner JD: Laser-induced fluorescence detection of a single molecule in a capillary. Anal Chem 66(23): 1142–4149, 1994.
  • 17
    Mets Ü, Rigler R: Submillisecond detection of single rhodamine molecules in water. J Fluorescence 4(3): 259–264, 1994.
  • 18
    Nguyen DC, Keller RA, Trkula M: Ultrasensitive laser-induced fluorescence detection in hydrodynamically focused flows. J Opt Soc Am B 4(2): 138–143, 1987.
  • 19
    Nie S, Chiu DT, Zare RN: Probing individual molecules with confocal fluorescence microscopy. Science 266: 1018–1021, 1994.
  • 20
    Petty JT, Johnson ME, Goodwin PM, Martin JC, Jett JH, Keller RA: Characterization of DNA size determination of small fragments by flow cytometry. Anal Chem 67: 1755–1761, 1995.
  • 21
    Press WH, Flannery BP, Teukolsky SA, Vetterling WT: Numerical Recipes in C. Cambridge University Press, Cambridge, 1988, pp 237–241.
  • 22
    Schecker JA, Goodwin PM, Affleck RL, Wu M, Martin JC, Jett JH, Keller RA: Flow-based continuous DNA sequencing via single molecule detection of enzymatically cleaved fluorescent nucleotides. In: Advanced Optical Methods for Ultrasensitive Detection, Proc. SPIE, Volume 2385, FeareyBL (ed.) The International Society for Optical Engineering, Bellingham, 1995, pp 4–12.
  • 23
    Shera EB, Seitzinger NK, Davis LM, Keller RA, Soper SA: Detection of single fluorescent molecules. Chem Phys Lett 174(6): 553–557, 1990.
  • 24
    Soper SA, Mattingly QL, Vegunta P: Photon burst detection of single near-infrared fluorescent molecules. Anal Chem 65: 740–747, 1993.
  • 25
    Soper SA, Shera EB, Martin JC, Jett JH, Hahn JH, Nutter HL, Keller RA: Single-molecule detection of rhodamine 6G in ethanolic solutions using continuous wave laser excitation. Anal Chem 63: 432–437, 1991.
  • 26
    Steen HB: Characteristics of flow cytometers. In: Flow Cytometry and Sorting, Ed. 2, MelamedMR, LindmoT, MendelsohnML (eds.) John Wiley & Sons, New York, 1990, pp 11–25.
  • 27
    Swerdlow H, Zhang JZ, Chen DY, Harke HR, Grey R, Wu S, Dovichi NJ: Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence. Anal Chem 63: 2835–2841, 1991.
  • 28
    UDT Sensors, Inc.: Optoelectronic Components Catalog. UDT Sensors, Inc., Hawthorne, CA, 1993, pp 5–7.
  • 29
    van den Engh G, Farmer C: Photo-bleaching and photon saturation in flow cytometry. Cytometry 13: 669–677, 1992.
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  • 30
    Wilkerson CW, Goodwin PM, Ambrose WP, Martin JC, Keller RA: Detection and lifetime measurement of single molecules in flowing sample streams by laser-induced fluorescence. Appl Phys Lett 62(17): 2030–2032, 1993.
  • 31
    Wu S, Dovichi NJ: High-sensitivity fluorescence detector for fluorescein isothiocyanate derivatives of amino acids separated by capillary zone electrophoresis. J Chromatogr 480: 141–155, 1989.
  • 32
    Zarrin F, Dovichi NJ: Sub-picoliter detection with the sheath flow cuvette. Anal Chem 57: 2690–2692, 1985.
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