Rapid detection of antimicrobial activity using flow cytometry
Article first published online: 8 MAR 2005
Copyright © 1995 Wiley-Liss, Inc.
Volume 21, Issue 4, pages 374–377, December 1995
How to Cite
Durodie, J., Coleman, K., Simpson, I. N., Loughborough, S. H. and Winstanley, D. W. (1995), Rapid detection of antimicrobial activity using flow cytometry. Cytometry, 21: 374–377. doi: 10.1002/cyto.990210409
- Issue published online: 23 JUN 2005
- Article first published online: 8 MAR 2005
- Manuscript Accepted: 30 JUN 1995
- Manuscript Received: 8 FEB 1995
- Flow cytometry;
- protein:cell size ratio;
- antimicrobial research
Different antibiotic classes vary considerably in their modes of action and hence in their effects on the bacterial cell. Flow cytometry was used to analyse E. coli cells treated with five antibiotics differing a their modes of action. The ratio of protein content, as measured by fluorescence, to forward light scatter (i.e., FL1:FSC) provided a simple and reliable way of detecting within 2 h of treatment antimicrobial activity at a 0.5 minimum inhibitory concentration. This ability to detect antimicrobial activity rapidly offers considerable advantages in drug research for the rapid detection of novel antimicrobials and may, with further development, find a use in the clinic for rapid susceptibility testing as an aid to the selection of therapy. © 1995 Wiley-Liss, Inc.