SEARCH

SEARCH BY CITATION

Keywords:

  • cytokine pretreatment;
  • neutrophil;
  • oxidative burst response;
  • N-formyl-L-methionyl-L-leucyl-phenylalanine receptor;
  • CD11b;
  • CD62L;
  • CD66b

Abstract

Background

Neutrophil activation is strongly related to organ dysfunction that occurs during systemic inflammatory responses. The aim of our study was to analyze the oxidative burst response in correlation to the up- and downregulation of N-formyl-L-methionyl-L-leucyl-phenylalanine (fMLP) receptors and the surface antigens CD11b, CD62L, and CD66b as potential surrogate markers of the degree of neutrophil priming for an increased oxidative burst response induced by proinflammatory cytokines.

Methods

Blood was taken from healthy donors. Neutrophils were pretreated with cytokines (interleukin [IL]–1β, IL-6, IL-8, granulocyte-macrophage colony-stimulating factor [GM-CSF], and tumor necrosis factor α [TNFα]; 0.01–10 ng/ml) and stimulated with fMLP (100 nM) in vitro. Functional and phenotypical parameters were quantified flow cytometrically.

Results

The oxidative burst response increased after priming with 0.1 ng/ml TNFα, 1 ng/ml GM-CSF, or 10 ng/ml IL-8. Upregulation of fMLP receptors, CD11b, and CD66b and downregulation of CD62L showed a close correlation to the oxidative burst response. Altered expression of these parameters partly reached significance at lower cytokine concentrations in comparison with the oxidative burst. IL-1β and IL-6 had no effect.

Conclusions

Our results showed that the expression of phenotypical parameters closely correlates with functional parameters in human neutrophils. Thus an up- or downregulation of antigens such as CD11b or CD62L reflects cytokine-induced functional changes. Cytometry Part A 57A:53–62, 2004. © 2003 Wiley-Liss, Inc.