Cytokine upregulation of surface antigens correlates to the priming of the neutrophil oxidative burst response
Article first published online: 23 DEC 2003
Copyright © 2004 Wiley-Liss, Inc.
Cytometry Part A
Volume 57A, Issue 1, pages 53–62, January 2004
How to Cite
Wittmann, S., Rothe, G., Schmitz, G. and Fröhlich, D. (2004), Cytokine upregulation of surface antigens correlates to the priming of the neutrophil oxidative burst response. Cytometry, 57A: 53–62. doi: 10.1002/cyto.a.10108
- Issue published online: 23 DEC 2003
- Article first published online: 23 DEC 2003
- Manuscript Accepted: 25 SEP 2003
- Manuscript Revised: 31 AUG 2003
- Manuscript Received: 13 APR 2003
- Forschungsförderprogramm ReForM of the University of Regensburg
- Deutsche Forschungsgemeinschaft. Grant Number: FR1165-1/2
- cytokine pretreatment;
- oxidative burst response;
- N-formyl-L-methionyl-L-leucyl-phenylalanine receptor;
Neutrophil activation is strongly related to organ dysfunction that occurs during systemic inflammatory responses. The aim of our study was to analyze the oxidative burst response in correlation to the up- and downregulation of N-formyl-L-methionyl-L-leucyl-phenylalanine (fMLP) receptors and the surface antigens CD11b, CD62L, and CD66b as potential surrogate markers of the degree of neutrophil priming for an increased oxidative burst response induced by proinflammatory cytokines.
Blood was taken from healthy donors. Neutrophils were pretreated with cytokines (interleukin [IL]–1β, IL-6, IL-8, granulocyte-macrophage colony-stimulating factor [GM-CSF], and tumor necrosis factor α [TNFα]; 0.01–10 ng/ml) and stimulated with fMLP (100 nM) in vitro. Functional and phenotypical parameters were quantified flow cytometrically.
The oxidative burst response increased after priming with 0.1 ng/ml TNFα, 1 ng/ml GM-CSF, or 10 ng/ml IL-8. Upregulation of fMLP receptors, CD11b, and CD66b and downregulation of CD62L showed a close correlation to the oxidative burst response. Altered expression of these parameters partly reached significance at lower cytokine concentrations in comparison with the oxidative burst. IL-1β and IL-6 had no effect.
Our results showed that the expression of phenotypical parameters closely correlates with functional parameters in human neutrophils. Thus an up- or downregulation of antigens such as CD11b or CD62L reflects cytokine-induced functional changes. Cytometry Part A 57A:53–62, 2004. © 2003 Wiley-Liss, Inc.