• 1
    Rogers AW. Techniques of autoradiography. Amsterdam: Elsevier; 1973.
  • 2
    Howard A, Pelc SR. Nuclear incorporation of P32 as demonstrated by autoradiographs. Exp Cell Res 1951; 2: 178187.
  • 3
    Quastler H, Sherman FG. Cell population kinetics in the intestinal epithelium of the mouse. Exp Cell Res 1959; 17: 420428.
  • 4
    Steel GG. Growth kinetics of tumours: cell population kinetics in relation to growth and treatment of cancer. Oxford: Clarendon Press; 1977.
  • 5
    Cleaver JE. Thymidine metabolism and cell kinetics. Amsterdam: North Holland; 1967.
  • 6
    Baserga R. The biology of cell reproduction. Cambridge, MA: Harvard University Press; 1985.
  • 7
    Caspersson T, Lomakka G. Scanning microscopy techniques for high resolution quantitative cytochemistry. Ann NY Acad Sci 1962; 97: 449463.
  • 8
    Kamentsky LA, Melamed MR, Derman, H. Spectrophotometer: new instrument for ultrarapid cell analysis. Science 1965; 150: 630631.
  • 9
    Dittrich W, Göhde W. Impulsfluorometrie bei Einzelzellen in Suspensionen. Z Naturforsch (B) 1969; 24b: 360361. [Ger]
  • 10
    Van Dilla MA, Trujillo TT, Mullaney PF, Coulter JR. Cell microfluorometry: a method for rapid fluorescence measurement. Science 1969; 163: 12131214.
  • 11
    Crissman HA, Mullany PF, Steinkamp JA. Methods and applications of flow systems for analysis and sorting of mammalian cells. Methods Cell Biol 1975; 9: 179246.
  • 12
    Tobey RA, Crissman HA. Use of flow microfluorometry in detailed analysis of the effects of chemical agents on cell cycle progression. Cancer Res 1972; 32: 27262732.
  • 13
    Göhde W, Dittrich W. Cytostatic effect of daunomycin in impulsecytophotometric test. [Die Cytostatische Wirkung von Daunomycin im Impulscytophotometrictest]. Arzneimittelforschung 1971; 21: 16561658. [Ger]
  • 14
    Dittrich W, Göhde W, Severin E, Reiffenstuhl G. The nucleus-cytoplasm relationship in the impulse cytophotometry of cervical and vaginal smears. 4th Meeting of the International Congress on Cytology, London, UK, 1971.
  • 15
    Crissman HA, Steinkamp JA. Rapid simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations. J Cell Biol 1973; 59: 766771.
  • 16
    Hudson B, Upholt WB, Divinny J, Vinogard, J. The use of an ethidium bromide analogue in the dye-density buoyant procedure for the isolation of closed circular DNA. Proc Natl Acad Sci USA 1969; 62: 813817.
  • 17
    Krishan A. A rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 1975; 66: 188113.
  • 18
    Vindeløv LL, Christensen IJ, Nissen NI. A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 1983; 3: 323327.
  • 19
    Crissman HA, Tobey RA. Cell cycle analysis in 20 minutes. Science 1974; 184; 12971298.
  • 20
    Dean PN, Jett JH. Mathematical analysis of DNA distributions derived from flow microfluorometry. J Cell Biol 1974; 60: 523527.
  • 21
    Barlogie B, Spitzer G, Hart JS, Johnston DA, Buchner T, Schuman J, Drewinko B. DNA histogram analysis of human hematopoietic cells. Blood 1976; 48: 245258.
  • 22
    Otto FJ, Oldiges H, Göhde W, Barlogie B, Schumann J. Flow cytogenetics of uncloned and cloned Chinese hamster cells. Cytogenet Cell Genet 1980; 27: 5256.
  • 23
    Jensen RH. Chromomycin A3 as a fluorescent probe for flow cytometry of human gynecological samples. J Histochem Cytochem 1977; 25: 573579.
  • 24
    Stöhr M, Eipel H, Goerttler K, Vogt-Schaden M. Extended application of flow microfluorometry by means of dual laser excitation. Histochemistry 1977; 51: 30315.
  • 25
    Göhde W, Schumann J, Zante J. The use of DAPI in pulse cytophotometry. In: LutzD, editor. Pulse cytophotometry. Ghent, Belgium: European Press; 1978. p 229232.
  • 26
    Darzynkiewicz Z, Traganos F, Kapuscinski J, Staiano-Coico L, Melamed MR. Accessibility of DNA in situ to various fluorochromes: relationship to chromatin changes during erythroid differentiation of Fried leukemia cells. Cytometry 1984; 5: 355367.
  • 27
    Lewalski H, Otto FJ, Kranert T, Wassmuth R. Flow cytometric detection of unbalanced ram spermatozoa from heterozygous 1;20 translocation carriers. Cytogenet Cell Genet 1993; 64: 286291.
  • 28
    Hilwig I. On the influence of the benzimidazol derivative (fluorochrome) on cell lines in tissue culture. Z Zellforsch Mikrosk Anat 1970; 104: 127134.
  • 29
    Arndt-Jovin DJ, Jovin TM. Analysis and sorting of living cells according to deoxyribonucleic acid content. J Histochem Cytochem 1977; 25: 585589.
  • 30
    Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T. A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry. J Immunol Methods 1999; 229: 131138.
  • 31
    Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 1983; 31: 13331335.
  • 32
    Jacobberger JW, Horan PK, Hare JD. Cell cycle analysis of asexual stages of erythrocytic malaria parasites. Cell Prolif 1992; 25: 431445.
  • 33
    Bagwell BC. Theoretical aspects of flow cytometry data analysis. In: BauerKE, DuqueRE, ShankeyTV, editors. Clinical flow cytometry: principles and applications. Baltimore: Williams & Wilkins; 1993. p 4161.
  • 34
    Fried J, Perez AG, Clarkson B. Quantitative analysis of cell cycle progression of synchronous cells by flow cytometry. Exp Cell Res 1980; 126: 6374.
  • 35
    Rabinovitch PS. Practical considerations for DNA content and cell cycle analysis. In: BauerKE, DuqueRE, ShankeyTV, editors. Clinical flow cytometry: principles and applications. Baltimore: Williams & Wilkins; 1993. p 117142.
  • 36
    Clinical guidelines for the use of tumor markers in breast and colorectal cancer. Adopted on May 17, 1996 by the American Society of Clinical Oncology. J Clin Oncol 1996; 14: 28432877.
  • 37
    Bagwell CB, Clark GM, Spyratos F, Chassevent A, Bendahl PO, Stal O, Killander D, Jourdan ML, Romain S, Hunsberger B, Baldetorp B. Optimizing flow cytometric DNA ploidy and S-phase fraction as independent prognostic markers for node-negative breast cancer specimens. Cytometry 2001; 46: 121135.
  • 38
    Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Lymphocyte stimulation: a rapid multiparameter analysis. Proc Natl Acad Sci USA 1976; 73: 28812884.
  • 39
    Darzynkiewicz Z, Sharpless T, Staiano-Coico L, Melamed MR. Subcompartments of the G1 phase of the cell cycle detected by flow cytometry. Proc Natl Acad Sci USA 1980; 77: 66966699.
  • 40
    Darzynkiewicz Z, Traganos F, Melamed MR. New cell cycle compartments identified by multiparameter flow cytometry. Cytometry 1980; 1: 98108.
  • 41
    Shapiro H. Flow cytometric estimation of DNA and RNA content in intact cells stained with Hoechst 33342 and pyronin Y. Cytometry 1981; 2: 143150.
  • 42
    Crissman HA, Darzynkiewicz Z, Tobey RA, Steinkamp JA. Correlated measurements of DNA, RNA, and protein in individual cells by flow cytometry. Science 1985; 228: 13211324.
  • 43
    Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR. Recognition of cells in mitosis by flow cytofluorometry. J Histochem Cytochem 1977; 25: 875880.
  • 44
    Latt SA. Detection of DNA synthesis in interphase nuclei by fluorescence microscopy. J Cell Biol 1974; 62: 546550.
  • 45
    Bohmer RM, Ellwart J. Cell cycle analysis by combining 5-bromodeoxyuridine/33258 Hoechst technique with DNA-specific ethidium bromide staining. Cytometry 1981; 2: 3134.
  • 46
    Kubbies M, Schindler D, Hoehn H, Rabinovitch PS. Cell cycle kinetics by BrdU-Hoechst flow cytometry: an alternative to the differential metaphase labelling technique. Cell Tissue Kinet 1985; 18: 551562.
  • 47
    Crissman HA, Steinkamp JA. A new method for rapid and sensitive detection of bromodeoxyuridine in DNA replication cells. Exp Cell Res 1987; 173: 256261.
  • 48
    Darzynkiewicz Z, Andreeff M, Traganos F, Sharpless T, Melamed MR. Discrimination of cycling and noncycling lymphocytes by BrdUrd-suppressed acridine orange fluorescence in a flow cytometric system. Exp Cell Res 1978; 115: 3135.
  • 49
    Darzynkiewicz Z, Traganos, F, Melamed MR. Distinction between 5-bromodeoxyuridine labeled and unlabeled mitotic cells by flow cytometry. Cytometry 1983; 3: 345348.
  • 50
    Gratzner HG. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 1982; 218: 474475.
  • 51
    Dolbeare F, Gratzner H, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci USA 1983; 80: 55735577.
  • 52
    Li X, Traganos F, Melamed MR, Darzynkiewicz Z. Detection of 5-bromo-2-deoxyuridine incorporated into DNA by labeling strand breaks induced by photolysis (SBIP). Int J Oncol 1994; 4: 11571161.
  • 53
    Gorczyca W, Bruno S, Darzynkiewicz RJ, Gong J, Darzynkiewicz Z. DNA strand breaks occurring during apoptosis: Their early in situ detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors. Int J Oncol 1992; 1: 639648.
  • 54
    Begg AC, McNally NJ, Shrieve DC, Karcher H. A method to measure duration of DNA synthesis and the potential doubling time from a single sample. Cytometry 1985; 6: 620626.
  • 55
    Terry NHA, White RA. Cell cycle kinetics estimated by analysis of bromodeoxyuridine incorporation. Methods Cell Biol 2001; 63: 355374.
  • 56
    Gerdes J, Schwab U, Lemke H, Stein H. Production of a mouse antibody reactive with a human nuclear antigen associated with proliferation. Int J Cancer 1983; 31: 1320.
  • 57
    Celis JE, Madsen P, Nielsen S, Celis A. Nuclear patterns of cyclin (PCNA) antigen distribution subdivide S-phase in cultured cells - some applications of PCNA antibodies. Leuk Res 1986; 10: 237249.
  • 58
    Cazzalini O, Perucca P, Riva F, Stivala LA, Bianchi L, Vannini V, Ducommun B, Prosperi E. p21 (CDKN1A) does not interfere with loading of PCNA at DNA replication sites but inhibits subsequent binding of DNA polymerase delta at the G1 phase transition. Cell Cycle 2003; 2: 596603.
  • 59
    Larsen JK, Landberg G, Roos G. Detection of proliferating cell nuclear antigen. Methods Cell Biol 2001; 63: 419431.
  • 60
    Bauer KD, Clevenger CV, Williams TJ, Epstein AL. Assessment of cell cycle-associated antigen expression using multiparameter flow cytometry and antibody-acridine orange sequential staining. J Histochem Cytochem 1986; 34: 245250.
  • 61
    Clevenger CV, Epstein AL, Bauer KD. Modulation of the nuclear antigen p105 as a function of cell cycle progression. J Cell Physiol 1987; 130: 336343.
  • 62
    Galderisi U, Jori FP, Giordano A. Cell cycle regulation and neural differentiation. Oncogene 2003; 22: 52085219.
  • 63
    Vermeulen K, Van Bockstaele DR, Berneman ZN. The cell cycle: a review of regulation, deregulation and therapeutic targets in cancer. Cell Prolif 2003; 36: 131149.
  • 64
    Gong J, Li X, Traganos F, Darzynkiewicz, Z. Expression of G1 and G2 cyclins measured in individual cells by multiparameter flow cytometry; a new tool in the analysis of the cell cycle. Cell Prolif 1994; 27: 357371.
  • 65
    Gong J, Ardelt B, Traganos F, Darzynkiewicz Z. Unscheduled expression of cyclin B1 and cyclin E in several leukemic and solid tumor cell lines. Cancer Res 1994; 54: 42854288.
  • 66
    Juan G, Gong J, Traganos F, Darzynkiewicz Z. Unscheduled expression of cyclins D1 and D3 in human tumor cell lines. Cell Prolif 1996; 29: 259266.
  • 67
    Darzynkiewicz Z, Gong J, Juan G, Ardelt, B, Traganos F. Cytometry of cyclin proteins. Cytometry 1996; 25: 113.
  • 68
    Jacobberger JW. Flow cytometric analysis of intracellular protein epitopes. In: StewartCC, NicholsonJKA, editors. Immunophenotyping. New York: Wiley-Liss; 2000. p 361407.
  • 69
    Katayama H, Brinkley WR, Sen S. The Aurora kinases: role in cell transformation and tumorigenesis. Cancer Metastasis Rev 2003; 22: 451464.
  • 70
    Juan G, Traganos F, James WM, Ray JM, Roberge M, Sauve DM, Anderson H, Darzynkiewicz Z. Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry 1998; 32: 7177.
  • 71
    Friedrich TD, Okubo E, Laffin J, Lehman JM. Okadaic acid induces appearance of the mitotic epitope MPM-2 in SV40-infected CV-1 cells with a >G2-phase DNA content. Cytometry 1998, 31: 260264.
  • 72
    den Elzen N, Pines J. Cyclin A is destroyed in prometaphase and can delay chromosome alignment and anaphase. J Cell Biol 2001; 153: 121136.
  • 73
    Parry DH, O'Farrell PH. The schedule of destruction of three mitotic cyclins can dictate the timing of events during exit from mitosis. Curr Biol 2001; 11: 671683;
  • 74
    Clute P, Pines J. Temporal and spatial control of cyclin B1 destruction in metaphase. Nat Cell Biol 1999; 1: 8287.
  • 75
    Juan G, Gruenwald S, Darzynkiewicz, Z. Phosphorylation of retinoblastoma susceptibility gene protein assayed in individual lymphocytes during their mitogenic stimulation. Exp Cell Res 1998; 239: 104110.
  • 76
    Huang X, Traganos F, Darzynkiewicz Z. DNA damage induced by DNA-topoisomerase I- and topoisomerase II- inhibitors detected by histone H2AX phosphorylation in relation to the cell cycle phase and apoptosis. Cell Cycle 2003; 2: 614619.
  • 77
    Jacobberger JW, Sramkowski RM, Frisa PS, Peng Ye P, Gottlieb MA, Hedley DW, Shankey TV, Smith BL, Paniagua M, Goolsby CL. Immunoreactivity of Stat5 phosphorylated on tyrosine as a cell-based measure of Bcr/Abl kinase activity. Cytometry 2003; 54A: 7588.
  • 78
    Kakino S, Sasaki K, Kurose A, Ito H. Intracellular localization of cyclin B1 during the cell cycle of glioma cells. Cytometry 1996; 24: 4954.
  • 79
    Deptala A, Bedner E, Gorczyca W, Darzynkiewicz Z. Simple assay of activation of nuclear factor kappa B (NF-κB) by laser scanning cytometry (LSC). Cytometry 1998; 33: 376382.
  • 80
    Juan G, Cordon-Cardo C. Intranuclear compartmentalization of cyclin E during the cell cycle: disruption of the nucleocytoplasm-nucleolar shuttling of cyclin E in bladder cancer. Cancer Res 2001; 61: 12201226.
  • 81
    Bedner E, Li X, Kunicki J, Darzynkiewicz Z. Translocation of Bax to mitochondria during apoptosis measured by laser scanning cytometry. Cytometry 2000; 41: 8388.