D cyclins in lymphocytes
Article first published online: 16 DEC 2004
Copyright © 2004 Wiley-Liss, Inc.
Cytometry Part A
Volume 63A, Issue 1, pages 1–9, January 2005
How to Cite
Kaplan, D., Meyerson, H., Husel, W., Lewandowska, K. and MacLennan, G. (2005), D cyclins in lymphocytes. Cytometry, 63A: 1–9. doi: 10.1002/cyto.a.20103
- Issue published online: 25 DEC 2004
- Article first published online: 16 DEC 2004
- Manuscript Accepted: 22 OCT 2004
- Manuscript Revised: 30 SEP 2004
- Manuscript Received: 9 JUL 2004
- National Institutes of Health. Grant Number: RO1-CA77428
- human lymphocytes;
- cellular proliferation;
- flow cytometry;
- D cyclins;
- subcellular localization;
D cyclins are essential for the progression of cells through the G1 phase of the cell cycle. There are three distinct D cyclins. Cyclin D1 has been shown to be expressed by many different types of cells but not by lymphocytes. Cyclins D2 and D3 have been found in lymphocytes.
We used high-resolution enzymatic amplification staining technology in conjunction with flow cytometry and confocal microscopy and with immunoblotting to reassess the expression of the D cyclins in human lymphocytes.
Using high-resolution technology for flow cytometry, we found all three D cyclins in quiescent human peripheral blood lymphocytes. Cyclin D1 was expressed in quiescent and activated cells at levels commensurate with those of actively proliferating tumor cell lines. Cyclin D1 was functional inasmuch as it was complexed with CDK4. In the quiescent cells, cyclin D1 was expressed in the cytoplasm but, after activation, was found in the nucleus.
These findings demonstrate that lymphocytes express cyclin D1 and necessitate a reappraisal of the hypothesis that the D cyclins subsume redundant activities with tissue-specific expression. © 2004 Wiley-Liss, Inc.