Part of this work was presented at the 10th Leipziger Workshop “Systems Biology and Clinical Cytomics,” April 7–9, 2005, Leipzig, Germany.
Sequential photobleaching of fluorochromes for polychromatic slide-based cytometry†
Article first published online: 14 FEB 2006
Copyright © 2006 International Society for Analytical Cytology
Cytometry Part A
Volume 69A, Issue 3, pages 139–141, March 2006
How to Cite
Mittag, A., Lenz, D., Bocsi, J., Sack, U., Gerstner, A. O. H. and Tárnok, A. (2006), Sequential photobleaching of fluorochromes for polychromatic slide-based cytometry. Cytometry, 69A: 139–141. doi: 10.1002/cyto.a.20227
- Issue published online: 22 FEB 2006
- Article first published online: 14 FEB 2006
- Manuscript Accepted: 2 MAY 2005
- Manuscript Revised: 1 MAY 2005
- Manuscript Received: 30 MAR 2005
- Interdisziplinäres Zentrum für klinsche Forschung (IZKF), Medical Faculty of the University of Leipzig. Grant Number: Z10
- slide-based cytometry;
- polychromatic cytometry
Slide-based cytometry is a key technology for polychromatic cytomic investigations. Here we exploit the relocalization and merge feature of Laser Scanning Cytometry for distinguishing fluorochromes of comparable emission spectra but different photostabilities.
Blood specimens were stained with the fluorochrome pairs: FITC/ALEXA488, PE/ALEXA532, or APC/ALEXA633. Bleaching was performed by repeated laser excitation.
Since ALEXA dyes are photostable as compared to the conventional fluorochromes FITC, PE, and APC, a differentiation within one fluorochrome pair is possible.
The sequential photobleaching method results in an increased information density on a single cell level and represents an important component to perform polychromatic cytometry. © 2006 International Society for Analytical Cytology