Part of this work was presented at the 10th Leipziger Workshop “Systems Biology and Clinical Cytomics”, April 7–9, 2005, Leipzig, Germany.
Laser scanning cytometry in human brain slices†
Version of Record online: 14 FEB 2006
Copyright © 2006 International Society for Analytical Cytology
Cytometry Part A
Volume 69A, Issue 3, pages 135–138, March 2006
How to Cite
Mosch, B., Mittag, A., Lenz, D., Arendt, T. and Tárnok, A. (2006), Laser scanning cytometry in human brain slices. Cytometry, 69A: 135–138. doi: 10.1002/cyto.a.20228
- Issue online: 22 FEB 2006
- Version of Record online: 14 FEB 2006
- Manuscript Accepted: 26 MAY 2005
- Manuscript Revised: 27 APR 2005
- Manuscript Received: 29 MAR 2005
- Saxon Ministry of Science and the Fine Arts
- Interdisciplinary Center for Clinical Research (IZKF) of the Medical faculty of the University of Leipzig, Germany. Grant Number: 01KS9504, project C1
- laser scanning cytometry;
- Alzheimer's disease;
- tissue sections;
- brain sections;
- cyclin B1;
- cell cycle analysis;
- two- and three-dimensional distribution
The Laser Scanning Cytometry (LSC) offers quantitative fluorescence analysis of cell suspensions and tissue sections.
We adapted this technique to immunohistochemical labelled human brain slices.
We were able to identify neurons according to their labelling and to display morphological structures such as the lamination of the entorhinal cortex. Further, we were able to distinguish between neurons with and without cyclin B1 expression and we could assign the expression of cyclin B1 to the cell islands of layer II and the pyramidal neurons of layer V of the entorhinal cortex in Alzheimer's disease effected brain. In addition, we developed a method depicting the three-dimensional distribution of the cells in intact tissue sections.
In this pilot experiments we could demonstrate the power of the LSC for the analysis of human brain sections. © 2006 International Society for Analytical Cytology