• 1
    De Rosa SC, Herzenberg LA, Roederer M. 11-color, 13-parameter flow cytometry: Identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat Med 2001; 7: 245248.
  • 2
    De Rosa SC, Brenchley JM, Roederer M. Beyond six colors: A new era in flow cytometry. Nat Med 2003; 9: 112117.
  • 3
    Kern F, LiPira G, Gratama JW, Manca F, Roederer M. Measuring Ag-specific immune responses: Understanding immunopathogenesis and improving diagnostics in infectious disease, autoimmunity and cancer. Trends Immunol 2005; 26: 477484.
  • 4
    Suni MA, Maino VC, Maecker HT. Ex vivo analysis of T-cell function. Curr Opin Immunol 2005; 17: 434440.
  • 5
    Perfetto SP, Chattopadhyay PK, Roederer M. Seventeen-colour flow cytometry: Unravelling the immune system. Nat Rev Immunol 2004; 4: 648655.
  • 6
    Sallusto F, Lenig D, Forster R, Lipp M, Lanzavecchia A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 1999; 401: 708712.
  • 7
    Marrack P, Kappler J. Control of T cell viability. Annu Rev Immunol 2004; 22: 765787.
  • 8
    Cossarizza A, Stent G, Mussini C, Paganelli R, Borghi V, Nuzzo C, Pinti M, Pedrazzi J, Benatti F, Esposito R, Rosok B, Nagata S, Vella S, Franceschi C, De Rienzo B. Deregulation of the CD95/CD95L system in lymphocytes from patients with primary acute HIV infection. AIDS 2000; 14: 345355.
  • 9
    Ligthart GJ, Corberand JX, Fournier C, Galanaud P, Hijmans W, Kennes B, Muller-Hermelink HK, Steinmann GG. Admission criteria for immunogerontological studies in man: The SENIEUR protocol. Mech Ageing Dev 1984; 28: 4755.
  • 10
    Gueresi P, Troiano L, Minicuci N, Bonafé M, Pini G, Salvioli G, Carani C, Ferrucci L, Spazzafumo L, Olivieri F, Cavrini G, Valentini D, Franceschi C. The MALVA (MAntova LongeVA) study: An investigation on people 98 years of age and over in a province of Northern Italy. Exp Gerontol 2003; 38: 11891197.
  • 11
    Eisen MB, Spellman PT, Brown PO, Botstein D. Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA 1998; 95: 1486314868.
  • 12
    Wold S, Esbensen K, Geladi P. Principal component analysis. Chemom Intell Lab Syst 1987; 2: 3752.
  • 13
    Nasi M, Troiano L, Lugli E, Pinti M, Ferraresi R, Monterastelli E, Mussi C, Salvioli G, Franceschi C, Cossarizza A. Thymic output and functionality of the IL-7/IL-7 receptor system in centenarians: Implications for the neolymphogenesis at the limit of human life. Aging Cell 2006; 5: 167175.
  • 14
    Perez OD, Nolan GP. Simultaneous measurement of multiple active kinase states using polychromatic flow cytometry. Nat Biotechnol 2002; 20: 155162.
  • 15
    Chattopadhyay PK, Yu J, Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles. Nat Med 2005; 11: 11131117.
  • 16
    Champagne P, Ogg GS, King AS, Knabenhans C, Ellefsen K, Nobile M, Appay V, Rizzardi GP, Fleury S, Lipp M, Forster R, Rowland-Jones S, Sekaly RP, McMichael AJ, Pantaleo G. Skewed maturation of memory HIV-specific CD8 T lymphocytes. Nature 2001; 410: 106111.
  • 17
    Brenchley JM, Karandikar NJ, Betts MR, Ambrozak DR, Hill BJ, Crotty LE, Casazza JP, Kuruppu J, Migueles SA, Connors M, Roederer M, Douek DC, Koup RA. Expression of CD57 defines replicative senescence and antigen-induced apoptotic death of CD8+ T cells. Blood 2003; 101: 27112720.
  • 18
    Curiel TJ, Coukos G, Zou L, Alvarez X, Cheng P, Mottram P, Evdemon-Hogan M, Conejo-Garcia JR, Zhang L, Burow M, Zhu Y, Wei S, Kryczek I, Daniel B, Gordon A, Myers L, Lackner A, Disis ML, Knutson KL, Chen L, Zou W. Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Nat Med 2004; 10: 942949.
  • 19
    Betts MR, Nason MC, West SM, De Rosa SC, Migueles SA, Abraham J, Lederman MM, Benito JM, Goepfert PA, Connors M, Roederer M, Koup RA. HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T-cells. Blood 2006; 107: 4781-4789.
  • 20
    Chattopadhyay PK, Price DA, Harper TF, Betts MR, Yu J, Gostick E, Perfetto SP, Goepfert P, Koup RA, De Rosa SC, Bruchez MP, Roederer M. Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry. Nat Med 2006; 12: 972977.
  • 21
    Hofmann M, Zerwes HG. Identification of organ-specific T cell populations by analysis of multiparameter flow cytometry data using DNA-chip analysis software. Cytometry A 2006; 69A: 533540.
  • 22
    Kim HR, Hong MS, Dan JM, Kang I. Altered IL-7Rα expression with aging and the potential implications of IL-7 therapy on CD8+ T-cell immune responses. Blood 2006; 107: 28552862.
  • 23
    Spaulding C, Guo W, Effros RB. Resistance to apoptosis in human CD8+ T cells that reach replicative senescence after multiple rounds of antigen-specific proliferation. Exp Gerontol 1999; 34: 633644.
  • 24
    Bryl E, Gazda M, Foerster J, Witkowski JM. Age-related increase of frequency of a new, phenotypically distinct subpopulation of human peripheral blood T cells expressing lowered levels of CD4. Blood 2001; 98: 11001107.
  • 25
    Bryl E, Vallejo AN, Weyand CM, Goronzy JJ. Down-regulation of CD28 expression by TNF-α. J Immunol 2001; 167: 32313238.
  • 26
    Salvioli S, Capri M, Scarcella E, Mangherini S, Faranca I, Volterra V, De Ronchi D, Marini M, Bonafe M, Franceschi C, Monti D. Age-dependent changes in the susceptibility to apoptosis of peripheral blood CD4+ and CD8+ T lymphocytes with virgin or memory phenotype. Mech Ageing Dev 2003; 124: 409418.
  • 27
    Pinti M, Troiano L, Nasi M, Bellodi C, Ferraresi R, Mussi C, Salvioli G, Cossarizza A. Balanced regulation of mRNA production for Fas and Fas ligand in lymphocytes from centenarians: How the immune system starts its second century. Circulation 2004; 110: 31083114.
  • 28
    Ginaldi L, De Martinis M, Monti D, Franceschi C. Chronic antigenic load and apoptosis in immunosenescence. Trends Immunol 2005; 26: 7984.