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cytoa20519-supplementaryfigure1.tif3519KSupplementary Figure 1: Gating strategies for quantification of GFAP-,β-3 tubulin-, and nestin-positive cells in undifferentiated and differentiated neurospheres. Following acquisition by flow cytometry data were processed by gating of side scatter against fluorescence signals. The figure shows plots of side scatter (SSC) versus forward scatter (FSC) signals and fluorescence signals versus SSC. The FSC parameter was used to exclude signals below channel 200 corresponding to cell debris.
cytoa20519-supplementaryfigure2.tif6049KSupplementary Figure 2: Expression of the components of the kallikrein-kinin system during neurosphere differentiation. Detection of mRNA transcription of high (HMW) and low molecular weight (LMW) kininogens and plasma kallikrein in neurospheres during differentiation of neurospheres to neurons, astrocytes and oligodendrocytes by RT-PCR. Provided are representative gel images of ethidium-bromide stained agarose gels (2 %) as original data. For quantification of sizes of gel fragments a 1 kb-plus DNA ladder (Invitrogen) (MW) was used. Und= Undifferentiated neurospheres; D1-D7: days of differentiation; RB= rat brain (positive control).
cytoa20519-Figure6-movieDay01.avi30724KSupporting Information file cytoa20519-Figure6-movieDay01.avi
cytoa20519-Figure6-movieDay03.avi34308KSupporting Information file cytoa20519-Figure6-movieDay03.avi
cytoa20519-Figure6-movieDay05.avi56326KSupporting Information file cytoa20519-Figure6-movieDay05.avi
cytoa20519-Figure6-movieDay07.avi56070KSupporting Information file cytoa20519-Figure6-movieDay07.avi

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