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Original Article
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Evaluation of intensity-based ratiometric FRET in image cytometry—Approaches and a software solution
Article first published online: 9 JUL 2009
DOI: 10.1002/cyto.a.20747
Copyright © 2009 International Society for Advancement of Cytometry
Additional Information
How to Cite
Roszik, J., Lisboa, D., Szöllősi, J. and Vereb, G. (2009), Evaluation of intensity-based ratiometric FRET in image cytometry—Approaches and a software solution. Cytometry, 75A: 761–767. doi: 10.1002/cyto.a.20747
Publication History
- Issue published online: 13 AUG 2009
- Article first published online: 9 JUL 2009
- Manuscript Accepted: 3 MAY 2009
- Manuscript Revised: 23 APR 2009
- Manuscript Received: 16 DEC 2008
Funded by
- European Community. Grant Numbers: EU FP6 LSHBCT-2004-503467, EU FP6 LSHC-CT-2005-018914, EU FP6 MRTN-CT-2005-019481
- Hungarian National Research Fund. Grant Numbers: K62648, K75752, K68763
LITERATURE CITED
- 1,. Imaging molecular interactions in living cells by FRET microscopy. Curr Opin Chem Biol 2006; 10: 409–416.
- 2,. FRET imaging. Nat Biotechnol 2003; 21: 1387–1395.
- 3,. Energy transfer: A spectroscopic ruler. Proc Natl Acad Sci USA 1967; 58: 719–726.
- 4,,. Microspectroscopic fluorescence analysis with prism-based imaging spectrometers: Review and current studies. Cytometry Part A 2006; 69A: 759–766.Direct Link:
- 5,,,. Hyperchromatic cytometry principles for cytomics using slide based cytometry. Cytometry A 2006; 69A: 691–703.Direct Link:
- 6,,,,,,,,. Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET). Biochem Soc Trans 2003; 31: 1020–1027.
- 7,,,,,,,. Two-sided fluorescence resonance energy transfer for assessing molecular interactions of up to three distinct species in confocal microscopy. Cytometry Part A 2008; 73A: 209–219.Direct Link:
- 8,,. Dissecting interacting molecular populations by FRET. Cytometry Part A 2008; 73A: 681–684.Direct Link:
- 9,. Fluorescence resonance energy transfer microscopy. In: CelisJE, editor. Cell Biology: A Laboratory Handbook. New York: Academic Press; 1998. pp 136–146.
- 10,,. Cytometry of fluorescence resonance energy transfer. Methods Cell Biol 2004; 75: 105–152.
- 11,,,,,. Enhanced FRET contrast in lifetime imaging. Cytometry Part A 2008; 73A: 745–753.Direct Link:
- 12,. Seeing through protein complexes by high-throughput FRET. Cytometry Part A 2008; 73A: 388–389.Direct Link:
- 13,,,,,. High throughput FRET screening of the plasma membrane based on TIRFM. Cytometry Part A 2008; 73A: 442–450.Direct Link:
- 14,. Photobleaching-corrected FRET efficiency imaging of live cells. Biophys J 2004; 86: 3923–3939.
- 15,,. Fluorescence resonance energy transfer-based stoichiometry in living cells. Biophys J 2002; 83: 3652–3664.
- 16,,,,. Measurement of FRET efficiency and ratio of donor to acceptor concentration in living cells. Biophys J 2006; 91: L39–L41.
- 17,,,,. Intensity-based energy transfer measurements in digital imaging microscopy. Eur Biophys J 1998; 27: 377–389.
- 18,,,,,,,. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem 1999; 47: 1179–1188.
- 19,,,,,,. Selecting the right fluorophores and flow cytometer for fluorescence resonance energy transfer measurements. Cytometry Part A 2005; 65A: 148–157.Direct Link:
- 20
- 21,,,. A new configuration of the Zeiss LSM 510 for simultaneous optical separation of green and red fluorescent protein pairs. Cytometry Part A 2006; 69A: 920–929.Direct Link:
- 22,,,,,,,,,. Novel calibration method for flow cytometric fluorescence resonance energy transfer measurements between visible fluorescent proteins. Cytometry Part A 2005; 67A: 86–96.Direct Link:
- 23,,,,. Cerulean, Venus, and VenusY67C FRET reference standards. Biophys J 2006; 91: L99–L101.
- 24. Available at: http://rsb.info.nih.gov/ij/.
- 25,,. AccPbFRET: An ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images. BMC Bioinformatics 2008; 9: 346.
- 26,,,,,,,,,. Conformation of the c-Fos/c-Jun complex in vivo: A combined FRET, FCCS, and MD-modeling study. Biophys J 2008; 94: 2859–2868.
- 27,,. The initiation of antigen-induced B cell antigen receptor signaling viewed in living cells by fluorescence resonance energy transfer. Nat Immunol 2005; 6: 1168–1176.
- 28RiFRET. Available at: http://www.biophys.dote.hu/rifret/.