Certain commercial equipment, instruments, or materials are identified in this paper to foster understanding and does not imply recommendation or endorsement by NIST, nor does it imply that the materials or equipment identified are necessarily the best available for the purpose.
Article first published online: 6 FEB 2010
Published 2010 Wiley-Liss, Inc.
Cytometry Part A
Volume 77A, Issue 8, pages 790–797, August 2010
How to Cite
Clarke, M. L., Burton, R. L., Hill, A. N., Litorja, M., Nahm, M. H. and Hwang, J. (2010), Low-cost, high-throughput, automated counting of bacterial colonies. Cytometry, 77A: 790–797. doi: 10.1002/cyto.a.20864
This article is a US government work and, as such, is in the public domain in the United States of America.
- Issue published online: 16 JUL 2010
- Article first published online: 6 FEB 2010
- Manuscript Accepted: 5 JAN 2010
- Manuscript Revised: 3 DEC 2009
- Manuscript Received: 22 OCT 2009
- PATH and NIH. Grant Number: N01 AI-30021
- colony counting;
Research involving bacterial pathogens often requires enumeration of bacteria colonies. Here, we present a low-cost, high-throughput colony counting system consisting of colony counting software and a consumer-grade digital camera or document scanner. We demonstrate that this software, called “NICE” (NIST's Integrated Colony Enumerator), can count bacterial colonies as part of a high-throughput multiplexed opsonophagocytic killing assay used to characterize pneumococcal vaccine efficacy. The results obtained with NICE correlate well with the results obtained from manual counting, with a mean difference of less than 3%. NICE is also rapid; it can count colonies from multiple reaction wells within minutes and export the results to a spreadsheet for data processing. As this program is freely available from NIST, NICE should be helpful in bacteria colony enumeration required in many microbiological studies, and in standardizing colony counting methods. Published 2010 Wiley-Liss, Inc.