Advances in cellular, subcellular, and nanoscale imaging in vitro and in vivo

Authors

  • Johannes T. Wessels,

    Corresponding author
    1. Department of Nephrology and Rheumatology, Molecular and Optical Live Cell Imaging, Center for Internal Medicine, University Medicine Goettingen, Göttingen, Germany
    • Department of Nephrology and Rheumatology, Molecular and Optical Live Cell Imaging, Center for Internal Medicine, University Medicine Goettingen, Göttingen, Germany
    Search for more papers by this author
    • Johannes T. Wessels and Fred S. Wouters contributed equally to the work.

  • Kensuke Yamauchi,

    1. AntiCancer Inc., San Diego, California
    2. Department of Surgery, University of California, San Diego, CA
    Search for more papers by this author
  • Robert M. Hoffman,

    1. AntiCancer Inc., San Diego, California
    2. Department of Surgery, University of California, San Diego, CA
    Search for more papers by this author
  • Fred S. Wouters

    1. Department of Neurophysiology, University Medicine, Göttingen, Germany
    2. CMPB, DFG Research Center Molecular Physiology of the Brain, Göttingen, Germany
    Search for more papers by this author
    • Johannes T. Wessels and Fred S. Wouters contributed equally to the work.


Abstract

This review focuses on technical advances in fluorescence microscopy techniques including laser scanning techniques, fluorescence-resonance energy transfer (FRET) microscopy, fluorescence lifetime imaging (FLIM), stimulated emission depletion (STED)-based super-resolution microscopy, scanning confocal endomicroscopes, thin-sheet laser imaging microscopy (TSLIM), and tomographic techniques such as early photon tomography (EPT) as well as on clinical laser-based endoscopic and microscopic techniques. We will also discuss the new developments in the field of fluorescent dyes and fluorescent genetic reporters that enable new possibilities in high-resolution and molecular imaging both in in vitro and in vivo. Small animal and tissue imaging benefit from the development of new fluorescent proteins, dyes, and sensing constructs that operate in the far red and near-infrared spectrum. © 2010 International Society for Advancement of Cytometry

Ancillary