Communicating subcellular distributions


  • Robert F. Murphy

    Corresponding author
    1. Lane Center for Computational Biology and Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania
    2. School of Life Sciences, Freiburg Institute for Advanced Studies, Albert Ludwig University of Freiburg, Freiburg im Breisgau, Germany
    • Carnegie Mellon University, 5000 Forbes Ave, Pittsburgh, Pennsylvania-15213, U.S.A
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To build more accurate models of cells and tissues, the ability to incorporate information on the distributions of proteins (and other macromolecules) will become increasingly important. This review describes current progress towards determining and representing protein subcellular patterns so that the information can be used as part of systems biology efforts. Approaches to decomposing an image of the subcellular pattern of a protein give critical information about the fraction of that protein in each of a number of fundamental patterns (e.g., organelles). Methods for learning generative models from images provide a means of capturing the essential properties and variation in those properties of cell shape and organelle patterns. The combination of models of fundamental patterns and vectors specifying the fraction of a protein in each of them provide a much better means of communicating subcellular patterns than the descriptive terms that are currently used. Communicating information about subcellular patterns is important not only for systems biology simulations but also for representing results from microscopy experiments, including high content screening and imaging flow cytometry, in a transportable and generalizable manner. © 2010 International Society for Advancement of Cytometry