This article is a US government work and, as such, is in the public domain in the United States of America.
Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix†
Article first published online: 13 JUL 2010
Published 2010 Wiley-Liss, Inc.
Cytometry Part A
Volume 77A, Issue 9, pages 895–903, September 2010
How to Cite
Peterson, A. W., Halter, M., Tona, A., Bhadriraju, K. and Plant, A. L. (2010), Using surface plasmon resonance imaging to probe dynamic interactions between cells and extracellular matrix. Cytometry, 77A: 895–903. doi: 10.1002/cyto.a.20938
- Issue published online: 23 AUG 2010
- Article first published online: 13 JUL 2010
- Manuscript Accepted: 7 JUN 2010
- Manuscript Revised: 3 JUN 2010
- Manuscript Received: 8 OCT 2009
- vascular smooth muscle cells;
- image analysis;
- surface plasmon resonance;
- cell spreading;
Spatially resolved details of the interactions of cells with a fibronectin modified surface were examined using surface plasmon resonance imaging (SPRI). SPRI is a label-free technique that is based on the spatial measurement of interfacial refractive index. SPRI is sensitive to short range interactions between cells and their substratum. The high contrast in SPR signal between cell edges and substratum facilitates identification of cell edges and segmentation of cell areas. With this novel technique, we demonstrate visualization of cell-substratum interactions, and how cell-substratum interactions change over time as cells spread, migrate, and undergo membrane ruffling. Published 2010 Wiley-Liss, Inc.