• Open Access

Minimum information about a flow cytometry experiment (MIFlowCyt) checklist (Numbered in accordance with MIFlowCyt 1.0 document)


  • 1Experiment Overview
    • 1.1Purpose
    • 1.2Keywords
    • 1.3Experiment Variables
    • 1.4Organization (name and address)
    • 1.5Primary Contact (name and email address)
    • 1.6Date (or time period)
    • 1.7Conclusions (if applicable)
    • 1.8Quality Control Measures
  • 2Flow Sample and Specimen Details
    • 2.1Sample/Specimen Material Description (include description, type, source, source treatment, taxonomy, age, gender, phenotype, genotype as applicable for biological samples; description and location for environmental samples)
    • 2.3Sample Treatment(s) Description
    • 2.4Fluorescence Reagent(s) Description (include characteristic(s) being measured, analytes, analyte detectors, analyte reporters, clone names/numbers, manufacturer, catalogue numbers as applicable)
  • 3Instrument Details
    • 3.1Instrument Manufacturer
    • 3.2Instrument Model
    • 3.3Instrument Configuration and Settings (provide acquisition settings including detector voltages and describe all custom alterations of the instrument if applicable; include installation dates of optical filters)
  • 4Data Analysis Details (if data analysis has been performed)
    • 4.1List-mode Data File (specify location of original list-mode file, for example supplementary material, URL, website)
    • 4.2Compensation Details (describe how multicolor compensation was performed by including antibodies, cells, or beads used)
    • 4.3Data Transformation Details (purpose and description if any transformation of the raw measurement has been performed, including various scales for visualization and gating purposes)
    • 4.4Gating (Data Filtering) Details (include description of all gates, percentage of events inside, and either mathematical descriptions of each gate boundary or appropriate gate images; mathematical description of gate boundaries can be provided in using Gating-ML or an appropriate project or workspace file); description of the algorithm by which gates were created (for example, subjective, based on FMO (how?), same gate for all analyses, etc.)
  • 5Data Presentation Requirements
    • 5.1Axes legends (antibody and dye; linear- or logarithmic-scaled axes)
    • 5.2Graphical example for full gating strategy
    • 5.3Positive/negative control or FMO

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