Irene Heimbeck, Thomas P. J. Hofer, Christiane Eder, Adam Wright, Ayman Marai, and Ghada Boghdadi performed experiments. Marion Frankenberger was involved in the study design and performed experiments. Jürgen Scherberich provided clinical samples. Loems Ziegler-Heitbrock was involved in the study design and wrote the article.
Standardized single-platform assay for human monocyte subpopulations: Lower CD14+CD16++ monocytes in females
Article first published online: 26 JUL 2010
Copyright © 2010 International Society for Advancement of Cytometry
Cytometry Part A
Volume 77A, Issue 9, pages 823–830, September 2010
How to Cite
Heimbeck, I., Hofer, T. P. J., Eder, C., Wright, A. K., Frankenberger, M., Marei, A., Boghdadi, G., Scherberich, J. and Ziegler-Heitbrock, L. (2010), Standardized single-platform assay for human monocyte subpopulations: Lower CD14+CD16++ monocytes in females. Cytometry, 77A: 823–830. doi: 10.1002/cyto.a.20942
- Issue published online: 23 AUG 2010
- Article first published online: 26 JUL 2010
- Manuscript Accepted: 11 JUN 2010
- Manuscript Revised: 9 JUN 2010
- Manuscript Received: 29 MAR 2010
- DFG. Grant Number: Zi 288
Additional Supporting Information may be found in the online version of this article.
|CYTO_20942_sm_suppfig1.tif||2129K||Figure S1 Isotype control staining for CD14 CD16 and DR. Given are the stainings that go along with the samples in Figure 1 in the main text. C1: CD14 versus DR histogram, C2: isotype-FITC versus DR histogram, C3: CD14 versus isotype-PC5 histogram, D1: CD14 versus CD16 histogram, D2: isotype-FITC versus CD16 histogram, D3: CD14 versus isotype-PE histogram. The data show proper setting of the instrument. For CD14 some overlap of weakly positive CD14+CD16++ monocytes with the isotype-FITC stained cells is seen (compare D1 and D2) and the same is true for HLA-DR expression on the CD14++CD16− monocytes when compared to isotype-PC5 stained cells (compare C1 and C3).|
|CYTO_20942_sm_suppfig2.tif||397K||Figure S2 Distribution of CD14+CD16++ monocyte numbers for females and males. The data show a reduced absolute number for females compared to males in all 4 age groups.|
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