The first two authors contributed equally to this work.
Original Article
You have free access to this content
Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples
Article first published online: 10 NOV 2010
DOI: 10.1002/cyto.a.20989
Copyright © 2010 International Society for Advancement of Cytometry
Additional Information
How to Cite
D'Alessio, F., Mirabelli, P., Gorrese, M., Scalia, G., Gemei, M., Mariotti, E., Di Noto, R., Martinelli, P., Fortunato, G., Paladini, D. and Del Vecchio, L. (2011), Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples. Cytometry, 79A: 14–24. doi: 10.1002/cyto.a.20989
- †
The first two authors contributed equally to this work.
Publication History
- Issue published online: 22 DEC 2010
- Article first published online: 10 NOV 2010
- Manuscript Accepted: 30 SEP 2010
- Manuscript Received: 11 MAY 2010
Funded by
- CEINGE-Biotecnologie Avanzate di Napoli Biobank
Literature Cited
- 1,,. New insights into cord blood stem cell transplantation. Curr Opin Hematol 2008; 15: 279–284.
- 2,,,,,. Identification of NCR+/NKG2D+/LFA-1low/CD94− immature human NK cell subset. Cytometry Part A 2009; 75A: 893–901.
- 3,,,,. Three-dimensional distribution patterns of CD34 antigen on nonactivated cord blood cells. Cytometry Part A 2008; 73A: 16–21.
- 4,,,,,,,. Characterization of banked umbilical cord blood hematopoietic progenitor cells and lymphocyte subsets and correlation with ethnicity, birth weight, sex, and type of delivery: A Cord Blood Transplantation (COBLT) Study report. Transfusion 2005; 45: 856–866.
- 5,,,,,,,,,,. Fetal cord blood as an alternative source of hematopoietic progenitor cells: Immunophenotype, maternal cell contamination, and ex vivo expansion. J Hematother 1999; 8: 141–155.
- 6,,,,,,. Preterm birth and the availability of cord blood for HPC transplantation. Transfusion 2000; 40: 817–820.
- 7,,,,,. Flow cytometric assessment of hematopoietic cell subsets in cryopreserved preterm and term cord blood, influence of obstetrical parameters, and availability for transplantation. Am J Hematol 2006; 81: 397–410.
- 8,,,,,,. Modern flow cytometry: A practical approach. Clin Lab Med 2007; 27: 453–468.
- 9,. Gestational age changes in circulating CD34+ hematopoietic stem/progenitor cells in fetal cord blood. Am J Obstet Gynecol 1998; 178: 931–937.
- 10,,,,. Quality assurance for polychromatic flow cytometry. Nat Protocols 2006; 1: 1522–1530.
- 11,,. Intracellular cytokine optimization and standard operating procedure. Nat Protocols 2006; 1: 1507–1516.
- 12,. Flow cytometry controls, instrument setup, and the determination of positivity. Cytometry Part A 2006; 69A: 1037–1042.
- 13,,,,. Interpreting flow cytometry data: A guide for the perplexed. Nat Immunol 2006; 7: 681–685.
- 14,,,,,. Immunophenotypic differentiation patterns of normal hematopoiesis in human bone marrow: Reference patterns for age-related changes and disease-induced shifts. Cytometry Part B 2004; 60B: 1–13.
- 15,. Enumeration of CD34+ cells in cord blood: A variation on a single-platform flow cytometric method based on the ISHAGE gating strategy. Cytometry 2001; 46: 254–261.
- 16,,,,,,,,. Umbilical cord blood from preterm human fetuses is rich in committed and primitive hematopoietic progenitors with high proliferative and self-renewal capacity. Exp Hematol 1999; 27: 1338–1345.
- 17,,. Cell-surface antigen expression in early and term gestation fetal hematopoietic progenitor cells. Stem Cells 1998; 16: 343–348.
- 18,,,,,,,. Immunophenotypic and functional characterization of CD33(+)CD34(+) cells in human cord blood of preterm neonates. Exp Hematol 2000; 28: 1174–1180.
- 19,,. Flow cytometry analysis of acute promyelocytic leukemia: The power of ‘surface hematology’. Leukemia 2007; 21: 4–8.
- 20,,,,,,,. Comparative characteristics of mesenchymal stem cells from human bone marrow and placenta: CD10, CD49d, and CD56 make a difference. Stem Cells Dev 2008; 17: 1039–1041.
- 21,. Cord blood banking: A historical perspective. Br J Haematol 2009; 147: 236–245.
- 22,,,,,,,,. Effects of long-term cryopreservation on hematopoietic progenitor cells in umbilical cord blood. Bone Marrow Transplant 1999; 23: 395–395.
- 23,,,,,,,. Identification and comparison of CD34-positive cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry. Blood 1991; 77: 2591–2596.
- 24,,,,. Human cord blood long-term engrafting cells are CD34+ CD38−. Leukemia 2003; 17: 960–964.
- 25. ABC transporters as phenotypic markers and functional regulators of stem cells. Stem Cells 2002; 20: 11–20.
- 26,,,,,,,,,,,,,,,. Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood 1997; 90: 111–125.
- 27,,,,,,,,,. Haematopoietic repopulating activity in human cord blood CD133(+) quiescent cells. Bone Marrow Transplant 2009; 43: 627–635.
- 28,,,,,,. Hematopoietic capacity of preterm cord blood hematopoietic stem/progenitor cells. Biochem Biophys Res Commun 2009; 389: 290–294.
- 29,,,,,,,,. Selection based on CD133 and high aldehyde dehydrogenase activity isolates long-term reconstituting human hematopoietic stem cells. Blood 2006; 107: 2162–2169.
- 30,. Regulation of hematopoiesis through adhesion receptors. JLeukoc Biol 2001; 69: 307–316.
- 31,,,,,,,,. Fetal haemopoietic cells display enhanced migration across endothelium. Br J Haematol 2002; 116: 392–400.
- 32,,. Fetal and adult hematopoietic stem cells require beta1 integrin function for colonizing fetal liver, spleen, and bone marrow. Immunity 2000; 12: 653–663.
- 33,,,,,,,,,,,,,. A novel role for PECAM-1 (CD31) in regulating haematopoietic progenitor cell compartmentalization between the peripheral blood and bone marrow. PLoS ONE 2008; 3: e2338.
- 34
- 35,,,,,,,. Human primitive hematopoietic progenitor cells are more enriched in KITlow cells than in KIThigh cells. Blood 1993; 82: 3283–3289.
- 36
- 37,,,,,,,. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatr 1997; 130: 388–393.
- 38,,,,. Fetal B lymphocyte subpopulations in normal pregnancies. Fetal Diagn Ther 1993; 8: 15–21.
- 39,. Development of human lymphoid cells. Annu Rev Immunol 2006; 24: 287–320.
- 40,. Fetal B-cell lymphopoiesis and the emergence of B-1-cell potential. Nat Rev Immunol 2007; 7: 213–219.

1552-4930/asset/olbannerleft.gif?v=1&s=cf6fcbfdc8ffa722551b71f8be0977b5b04db6ee)
1552-4930/asset/olbannerright.gif?v=1&s=6d0ba4e64946e5d9d4ebba4b8395a68bf324cd0b)
