SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
CYTO_21022_sm_suppfig1.tif213KSupporting Figure 1 Gating strategy for kinetic analysis of mmp and pmp of live cells. A cell gate was applied using the forward and side scatter parameters, FSC and SSC respectively A). A live cell gate was applied to a annexin VFITC v DAPI dot-plot as indicated by the black box gating on annexin V-FITC-ve/ DAPI-ve events B). A kinetic overlay of the bis-oxonol 610/10nm and DiIC1(5) 660/20nm signals onto the same y axis was generated using FlowJo software (Treestar Inc. CA) C).
CYTO_21022_sm_suppfig2.tif690KSupporting Figure 2 Annexin V binding assay. Jurkat T cells treated with 1 μM staurosporine (STS) to induce apoptosis A), 1% sodium azide B), heat shock 42oC C), 0.01% Triton X-100 D) for 24 h. Cells were loaded with DiIC1(5) and bis-oxonol and labeled with annexin V-FITC and DAPI. 100,000 events were collected. Data analysis of Annexin V versus DAPI dot-plots for each treatment were plotted to show the degree of cell death and annexin V binding respectively. Dot-plots representative experiments, n=3.
CYTO_21022_sm_suppfig3.tif126KSupporting Figure 3 Sub G1 analysis was performed on fixed cells after 24h treatment and stained with propidium iodide (DNA content). The percent of cell in Sub G1 are indicate for each treatment, A) control cells B) STS C) heat shock at 42oC D) 1% Sodium Azide and E) 0.01% Triton X-100.
CYTO_21022_sm_suppfig4.tif137KSupporting Figure 4 Plasma membrane depolarization on live cells after 24h of treatment. Jurkat T cells were untreated (control) A) or treated with 1 μM staurosporine, apoptosis B), heat shock at 42oC C), 1% sodium azide D) and 0.01% Triton X-100 E) for 24 h. Cells were loaded with DiIC1(5) and bis-oxonol and labeled with annexin V-FITC and DAPI. 100,000 events were collected and data analysed to show changes in bis-oxonol fluorescence. Live cells (annexinV-ve/DAPI-ve), were analysed for bis-oxonol depolarisation. Histograms are of representative experiments, n=3.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.