D.G. and U.S. equally contributed to this publication.
Increased CD64 expression on polymorphonuclear neutrophils indicates infectious complications following solid organ transplantation†
Article first published online: 6 APR 2011
Copyright © 2011 International Society for Advancement of Cytometry
Cytometry Part A
Volume 79A, Issue 6, pages 446–460, June 2011
How to Cite
Grey, D., Sack, U., Scholz, M., Knaack, H., Fricke, S., Oppel, C., Luderer, D., Fangmann, J., Emmrich, F. and Kamprad, M. (2011), Increased CD64 expression on polymorphonuclear neutrophils indicates infectious complications following solid organ transplantation. Cytometry, 79A: 446–460. doi: 10.1002/cyto.a.21049
Partial results have been presented in 2008 at the 13th Workshop Cytomics and NanoBioengineering in Leipzig, the DGfI Annual Meeting in Vienna, the “DGFZ/ESCCA joint meeting” in Bremen and the 5th DGKL Annual Meeting in Mannheim.
- Issue published online: 18 MAY 2011
- Article first published online: 6 APR 2011
- Manuscript Accepted: 17 FEB 2011
- Manuscript Revised: 14 FEB 2011
- Manuscript Received: 30 MAY 2010
- IQ Products, Groningen, The Netherlands, the Translational Center for Regenerative Medicine (TRM) Leipzig, Leipzig, Germany
- PMN CD64;
- solid organ transplantation;
The aim of this study was to evaluate the diagnostic value of monitoring CD64 antigen upregulation on polymorphonuclear neutrophils (PMN) for the identification of infectious complications in the postoperative course of solid organ transplanted patients. Twenty-five kidney, 13 liver, and four pancreas–kidney transplanted patients were included. Beginning with preoperative values up to postoperative values after 3 months for each patient, the PMN CD64 Index, HLA-DR on monocytes, NKp44+ NK and NK/T cells, CXCR3+ NK cells, CXCR3+ T helper cells, CXCR3+ NK/T cells, and CD4/CD8 ratio were measured by flow cytometry. Subsequently they were correlated with confirmed postoperative complications. Measuring the PMN CD64 Index reached a sensitivity of 89% and a specificity of 65% in the detection of infectious complications. Concerning this matter, it was a significantly better marker than all other included parameters except CXCR3+ NK/T cells. In contrast, according to our results the PMN CD64 Index has no diagnostic relevance in detection of rejections. The combination of included parameters showed no improved diagnostic value. Due to its high sensitivity and specificity for infectious complications CD64 on PMN could be proven a very good indicator in evaluating suspected infectious complications in the postoperative course of transplanted patients. © 2011 International Society for Advancement of Cytometry.