Increased CD64 expression on polymorphonuclear neutrophils indicates infectious complications following solid organ transplantation

Authors

  • Daniel Grey,

    Corresponding author
    1. Institute of Clinical Immunology, Medical Faculty, Universität Leipzig, Leipzig, Germany
    • Institute of Clinical Immunology, Medical Faculty, Universität Leipzig AöR, Johannisallee 30, Leipzig 04103, Germany
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  • Ulrich Sack,

    1. Institute of Clinical Immunology, Medical Faculty, Universität Leipzig, Leipzig, Germany
    2. Translational Centre for Regenerative Medicine (TRM) Leipzig, Universität Leipzig, Leipzig, Germany
    3. Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany
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    • D.G. and U.S. equally contributed to this publication.

  • Markus Scholz,

    1. Institute for Medical Informatics, Statistics, and Epidemiology, Medical Faculty, Universität Leipzig, Leipzig, Germany
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  • Heike Knaack,

    1. Institute of Clinical Immunology, Medical Faculty, Universität Leipzig, Leipzig, Germany
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  • Stephan Fricke,

    1. Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany
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  • Christoph Oppel,

    1. Institute of Clinical Immunology, Medical Faculty, Universität Leipzig, Leipzig, Germany
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  • Daniel Luderer,

    1. Institute of Clinical Immunology, Medical Faculty, Universität Leipzig, Leipzig, Germany
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  • Josef Fangmann,

    1. Department for Visceral-Surgery, Karl-Olga-Hospital, Stuttgart, Germany
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  • Frank Emmrich,

    1. Institute of Clinical Immunology, Medical Faculty, Universität Leipzig, Leipzig, Germany
    2. Translational Centre for Regenerative Medicine (TRM) Leipzig, Universität Leipzig, Leipzig, Germany
    3. Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany
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  • Manja Kamprad

    1. Institute of Clinical Immunology, Medical Faculty, Universität Leipzig, Leipzig, Germany
    2. Translational Centre for Regenerative Medicine (TRM) Leipzig, Universität Leipzig, Leipzig, Germany
    3. Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany
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  • Partial results have been presented in 2008 at the 13th Workshop Cytomics and NanoBioengineering in Leipzig, the DGfI Annual Meeting in Vienna, the “DGFZ/ESCCA joint meeting” in Bremen and the 5th DGKL Annual Meeting in Mannheim.

Abstract

The aim of this study was to evaluate the diagnostic value of monitoring CD64 antigen upregulation on polymorphonuclear neutrophils (PMN) for the identification of infectious complications in the postoperative course of solid organ transplanted patients. Twenty-five kidney, 13 liver, and four pancreas–kidney transplanted patients were included. Beginning with preoperative values up to postoperative values after 3 months for each patient, the PMN CD64 Index, HLA-DR on monocytes, NKp44+ NK and NK/T cells, CXCR3+ NK cells, CXCR3+ T helper cells, CXCR3+ NK/T cells, and CD4/CD8 ratio were measured by flow cytometry. Subsequently they were correlated with confirmed postoperative complications. Measuring the PMN CD64 Index reached a sensitivity of 89% and a specificity of 65% in the detection of infectious complications. Concerning this matter, it was a significantly better marker than all other included parameters except CXCR3+ NK/T cells. In contrast, according to our results the PMN CD64 Index has no diagnostic relevance in detection of rejections. The combination of included parameters showed no improved diagnostic value. Due to its high sensitivity and specificity for infectious complications CD64 on PMN could be proven a very good indicator in evaluating suspected infectious complications in the postoperative course of transplanted patients. © 2011 International Society for Advancement of Cytometry.

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