Original Article

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Optimal reprogramming factor stoichiometry increases colony numbers and affects molecular characteristics of murine induced pluripotent stem cells†‡

  1. Ulf Tiemann1,2,
  2. Malte Sgodda1,
  3. Eva Warlich3,4,
  4. Matthias Ballmaier5,
  5. Hans R. Schöler1,2,6,
  6. Axel Schambach3,4,§,
  7. Tobias Cantz1,2,*

Article first published online: 4 MAY 2011

DOI: 10.1002/cyto.a.21072

Issue

Cytometry Part A

Cytometry Part A

Volume 79A, Issue 6, pages 426–435, June 2011

Additional Information

How to Cite

Tiemann, U., Sgodda, M., Warlich, E., Ballmaier, M., Schöler, H. R., Schambach, A. and Cantz, T. (2011), Optimal reprogramming factor stoichiometry increases colony numbers and affects molecular characteristics of murine induced pluripotent stem cells. Cytometry, 79A: 426–435. doi: 10.1002/cyto.a.21072

Author Information

  1. 1

    Junior Research Group Stem Cell Biology, Cluster of Excellence REBIRTH, Hannover Medical School, 30625 Hannover, Germany

  2. 2

    Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany

  3. 3

    Junior Research Group Hematopoietic Cell Therapy, Cluster of Excellence REBIRTH, Hannover Medical School, 30625 Hannover, Germany

  4. 4

    Department of Experimental Hematology, Hannover Medical School, 30625 Hannover, Germany

  5. 5

    Cell Sorting Core Facility, Hannover Medical School, 30625 Hannover, Germany

  6. 6

    Medical Faculty, University of Münster, 48149 Münster, Germany

  1. §

    Contact for plasmid requests

*Stem Cell Biology, Cluster of Excellence REBIRTH, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany

  1. †

    Author Disclosure Statement: The authors declare that no conflicting financial interests exist.

  2. ‡

    Author contributions: U.T.: vector production, iPSC generation and characterization, data analysis and interpretation, and manuscript writing. M.S.: qRT-PCR analyses. E.W.: generation of vector plasmids. M.B.: flow cytometry setup and cell sorting. H.R.S.: conception and design. A.S.: generation of vector plasmids and design of the study. T.C.: conceptional design of the study, data analysis and interpretation, and manuscript writing. All authors approved the final version of the manuscript.

Publication History

  1. Issue published online: 18 MAY 2011
  2. Article first published online: 4 MAY 2011
  3. Manuscript Accepted: 4 APR 2011
  4. Manuscript Revised: 25 MAR 2011
  5. Manuscript Received: 2 FEB 2011

Funded by

  • German Research Foundation. Grant Number: DFG: EXC 62/1
  • German Ministry for Education and Research. Grant Number: 01GN0812, 01GM0854 and ReGene
  • DAAD. Grant Number: 0315187

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