Cell labeling approaches for fluorescence-based in vivo flow cytometry

Authors

  • Costas M. Pitsillides,

    1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
    2. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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    • Costas M. Pitsillides and Judith M. Runnels contributed equally to this work.

  • Judith M. Runnels,

    1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
    2. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Joel A. Spencer,

    1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
    2. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Liang Zhi,

    1. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Mei X. Wu,

    1. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Charles P. Lin

    Corresponding author
    1. Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
    2. Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
    • PhD, Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114
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Abstract

We provide an overview of the methods used to label circulating cells for fluorescence detection by in vivo flow cytometry. These methods are useful for cell tracking in small animals without the need to draw blood samples and are particularly useful for the detection of circulating cancer cells and quantification of circulating immune cells. © 2011 International Society for Advancement of Cytometry.

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