Flow cytometry as a tool for analyzing changes in Plasmodium falciparum cell cycle following treatment with indol compounds

Authors

  • Desirée Cigaran Schuck,

    1. Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil
    2. Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-90, Brazil
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  • Ramira Yuri Ribeiro,

    1. Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil
    2. Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-90, Brazil
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  • Arthur A. Nery,

    1. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, Brazil
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  • Henning Ulrich,

    1. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, Brazil
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  • Célia R. S. Garcia

    Corresponding author
    1. Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil
    2. Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-90, Brazil
    • Rua do Matão 101, Travessa 14, São Paulo, SP, 05508-900, Brazil
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Abstract

Melatonin and its derivatives modulate the Plasmodium falciparum and Plasmodium chabaudi cell cycle. Flow cytometry was employed together with the nucleic acid dye YOYO-1 allowing precise discrimination between mono- and multinucleated forms of P. falciparum-infected red blood cell. The use of YOYO-1 permitted excellent discrimination between uninfected and infected red blood cells as well as between early and late parasite stages. Fluorescence intensities of schizont-stage parasites were about 10-fold greater than those of ring-trophozoite form parasites. Melatonin and related indolic compounds including serotonin, N-acetyl-serotonin and tryptamine induced an increase in the percentage of multinucleated forms compared to non-treated control cultures. YOYO-1 staining of infected erythrocyte and subsequent flow cytometry analysis provides a powerful tool in malaria research for screening of bioactive compounds. © 2011 International Society for Advancement of Cytometry

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