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Flow cytometry as a tool for analyzing changes in Plasmodium falciparum cell cycle following treatment with indol compounds

  1. Desirée Cigaran Schuck1,2,
  2. Ramira Yuri Ribeiro1,2,
  3. Arthur A. Nery3,
  4. Henning Ulrich3,
  5. Célia R. S. Garcia1,2,*

Article first published online: 30 AUG 2011

DOI: 10.1002/cyto.a.21136

Issue

Cytometry Part A

Cytometry Part A

Volume 79A, Issue 11, pages 959–964, November 2011

Additional Information

How to Cite

Schuck, D. C., Ribeiro, R. Y., Nery, A. A., Ulrich, H. and Garcia, C. R. S. (2011), Flow cytometry as a tool for analyzing changes in Plasmodium falciparum cell cycle following treatment with indol compounds. Cytometry, 79A: 959–964. doi: 10.1002/cyto.a.21136

Author Information

  1. 1

    Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil

  2. 2

    Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-90, Brazil

  3. 3

    Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo 05508-900, Brazil

*Rua do Matão 101, Travessa 14, São Paulo, SP, 05508-900, Brazil

Publication History

  1. Issue published online: 19 OCT 2011
  2. Article first published online: 30 AUG 2011
  3. Manuscript Accepted: 4 AUG 2011
  4. Manuscript Revised: 28 JUL 2011
  5. Manuscript Received: 19 APR 2011

Funded by

  • FAPESP. Grant Number: 07/52924-0
  • Malaria Pronex
  • INCT-INBqMed
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
  • CAPES

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Keywords:

  • Plasmodium falciparum;
  • melatonin;
  • cell cycle;
  • flow cytometry

Abstract

Melatonin and its derivatives modulate the Plasmodium falciparum and Plasmodium chabaudi cell cycle. Flow cytometry was employed together with the nucleic acid dye YOYO-1 allowing precise discrimination between mono- and multinucleated forms of P. falciparum-infected red blood cell. The use of YOYO-1 permitted excellent discrimination between uninfected and infected red blood cells as well as between early and late parasite stages. Fluorescence intensities of schizont-stage parasites were about 10-fold greater than those of ring-trophozoite form parasites. Melatonin and related indolic compounds including serotonin, N-acetyl-serotonin and tryptamine induced an increase in the percentage of multinucleated forms compared to non-treated control cultures. YOYO-1 staining of infected erythrocyte and subsequent flow cytometry analysis provides a powerful tool in malaria research for screening of bioactive compounds. © 2011 International Society for Advancement of Cytometry

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