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Review Article
Recent advances in dynamic intravital multi-photon microscopy
Article first published online: 8 SEP 2011
DOI: 10.1002/cyto.a.21140
Copyright © 2011 International Society for Advancement of Cytometry
Issue
Cytometry Part A
Special Issue: In Vivo Flow Cytometry
Volume 79A, Issue 10, pages 789–798, October 2011
Additional Information
How to Cite
Niesner, R. A. and Hauser, A. E. (2011), Recent advances in dynamic intravital multi-photon microscopy. Cytometry, 79A: 789–798. doi: 10.1002/cyto.a.21140
Publication History
- Issue published online: 20 SEP 2011
- Article first published online: 8 SEP 2011
- Manuscript Accepted: 13 AUG 2011
- Manuscript Revised: 11 AUG 2011
- Manuscript Received: 4 APR 2011
Keywords:
- multi-photon laser scanning microscopy;
- intravital imaging
Abstract
Standard multiphoton laser scanning microscopy (MPLSM) has revolutionized our view of physiologic and pathologic processes in living organisms by enlightening different aspects of cellular choreography in immune responses, that is, cellular motility and co-localization. To understand cellular communication on a molecular level, novel transgenic reporter mice have been generated. In parallel, MPLSM systems have been developed, which make it possible for this technique to be more widely used to address crucial immunological questions. Here, we review the latest progress concerning transgenic mouse technology and multiphoton imaging capacities and discuss further developments which will enable us to visualize all around monitoring and quantification of cellular function at a molecular level directly in vivo. © 2011 International Society for Advancement of Cytometry