In vivo flow cytometry: A horizon of opportunities

Authors

  • Valery V. Tuchin,

    Corresponding author
    1. Research-Educational Institute of Optics and Biophotonics, Saratov State University, Saratov 410012, Russia
    2. Institute of Precise Mechanics and Control, Russian Academy of Sciences, Saratov 410028, Russia
    3. University of Oulu, Oulu, FI-90014, Finland
    • Saratov State University, Saratov 410012, Russia
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  • Attila Tárnok,

    Corresponding author
    1. Pediatric Cardiology, Heart Center, University of Leipzig, Leipzig D-04289, Germany
    • Department of Pediatric Cardiology, Heart Centre Leipzig, University of Leipzig, Strumpellstr. 39, 04289 Leipzig, Germany
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  • Vladimir P. Zharov

    Corresponding author
    1. Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205
    • Phillips Classic Laser and Nanomedicine Laboratories, University of Arkansas for Medical Sciences, Little Rock, AR 72205
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Abstract

Flow cytometry (FCM) has been a fundamental tool of biological discovery for many years. Invasive extraction of cells from a living organism, however, may lead to changes in cell properties and prevents studying cells in their native environment. These problems can be overcome by use of in vivo FCM, which provides detection and imaging of circulating normal and abnormal cells directly in blood or lymph flow. The goal of this review is to provide a brief history, features, and challenges of this new generation of FCM methods and instruments. Spectrum of possibilities of in vivo FCM in biological science (e.g., cell metabolism, immune function, or apoptosis) and medical fields (e.g., cancer, infection, and cardiovascular disorder) including integrated photoacoustic-photothermal theranostics of circulating abnormal cells are discussed with focus on recent advances of this new platform. © 2011 International Society for Advancement of Cytometry

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