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Analysis of nucleo-cytoplasmic shuttling of the proto-oncogene SET/I2PP2A

  1. B. Daniel Lam,
  2. Eloise C. Anthony,
  3. Peter L. Hordijk*

Article first published online: 11 OCT 2011

DOI: 10.1002/cyto.a.21153

Issue

Cytometry Part A

Cytometry Part A

Volume 81A, Issue 1, pages 81–89, January 2012

Additional Information

How to Cite

Daniel Lam, B., Anthony, E. C. and Hordijk, P. L. (2012), Analysis of nucleo-cytoplasmic shuttling of the proto-oncogene SET/I2PP2A. Cytometry, 81A: 81–89. doi: 10.1002/cyto.a.21153

Author Information

  1. Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, 1066 CX Amsterdam, The Netherlands

*Department of Molecular Cell Biology, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Plesmanlaan 125, 1066 CX Amsterdam, The Netherlands

Publication History

  1. Issue published online: 15 DEC 2011
  2. Article first published online: 11 OCT 2011
  3. Manuscript Accepted: 16 SEP 2011
  4. Manuscript Revised: 26 JUL 2011
  5. Manuscript Received: 10 MAY 2011

Funded by

  • Sanquin. Grant Number: 07-021 (PPOC project)

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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
CYTO_21153_sm_SuppMov1.avi1398KSupplementary Movie 1. (1.36 MB): Movie of HeLa cells transfected with YFP-SET(wt) (A) or mCherry-H2B (B), showing the maximum intensity projection in time, corresponding to the stills of Figure 1B. In two of the four cells, a SET nuclear exit-event is recorded. Movie spans 20 hours, recorded with a time interval of 5 minutes, and runs at 10 images per second.
CYTO_21153_sm_SuppMov2.avi768KSupplementary Movie 2. (767 KB): Movie of YFP-SET(wt) in HeLa cells spreading on fibronectin and corresponding to the data in Figure 2A. Shortly after the cell is fully spread, SET exits the nucleus. Movie spans 44 minutes, recorded with a time interval of 1 minute, and runs at 10 images per second.
CYTO_21153_sm_SuppMov3.avi1107KSupplementary Movie 3. (1.08 MB): Movie of YFP-SET(wt) translocation in dividing HeLa cells, corresponding to the data in Figure 2B. Just before cell division SET exits the nucleus (arrow). After division, in one of the daughter cells another nuclear exit-event of SET is recorded, in the other cell, SET remains nuclear. Movie spans 2 hours and 40 minutes, recorded with a time interval of 1 minute, and runs at 10 images per second.
CYTO_21153_sm_SuppMov4.avi412KSupplementary Movie 4. (412 KB): Movie of YFP-SET(wt) exiting the nucleus in one selected cell after thresholding, corresponding with the trace of Figure 4B. A single exit event is seen. Movie spans 20 hours, recorded with a time interval of 5 minutes, and runs at 10 images per second.
CYTO_21153_sm_SuppMov5.avi477KSupplementary Movie 5. (476 KB): Movie of YFP-SET(wt) exiting the nucleus, corresponding with the trace of Figure 4D. As the exit is partial, three exit events can be recorded. Movie spans 20 hours, recorded with a time interval of 5 minutes, and runs at 10 images per second.
CYTO_21153_sm_SuppMov1BH2B.avi1545KSupplementary Movie 1.

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